Validation Data Gallery
Tested Applications
| Positive WB detected in | A549 cells, Jurkat cells, MCF-7 cells, MDA-MB-231 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
27617-1-AP targets TSPYL5 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag26314 Product name: Recombinant human TSPYL5 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-210 aa of BC045630 Sequence: MSGRSRGRKSSRAKNRGKGRAKARVRPAPDDAPRDPDPSQYQSLGEDTQAAQVQAGAGWGGLEAAASAQLLRLGEEAACRLPLDCGLALRARAAGDHGQAAARPGPGKAASLSERLAADTVFVGTAGTVGRPKNAPRVGNRRGPAGKKAPETCSTAGRGPQVIAGGRQKKGAAGENTSVSAGEEKKEERDAGSGPPATEGSMDTLENVQL 相同性解析による交差性が予測される生物種 |
| Full Name | TSPY-like 5 |
| Calculated molecular weight | 417 aa, 45 kDa |
| Observed molecular weight | 45-50 kDa |
| GenBank accession number | BC045630 |
| Gene Symbol | TSPYL5 |
| Gene ID (NCBI) | 85453 |
| RRID | AB_3742244 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q86VY4 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
TSPYL5 (Testis-specific Y-encoded-like protein 5) is involved in modulation of cell growth and cellular response to gamma radiation probably via regulation of the Akt signaling pathway. It is also involved in the regulation of p53/TP53, and suppresses p53/TP53 protein levels and promotes its ubiquitination; Its function is dependent on USP7 and independent on MDM2 (PMID: 20079711; 21170034).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for TSPYL5 antibody 27617-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |