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Validation Data Gallery

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  • Western Blot (WB) analysis of human testis tissue using TSPY3 Polyclonal antibody (22257-1-AP)

    WB analysis of human testis using 22257-1-AP (same clone as 22257-1-PBS)

    human testis tissue were subjected to SDS PAGE followed by western blot with 22257-1-AP (TSPY3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22257-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of LNCaP cells using TSPY3 Polyclonal antibody (22257-1-AP)

    WB analysis of LNCaP using 22257-1-AP (same clone as 22257-1-PBS)

    LNCaP cells were subjected to SDS PAGE followed by western blot with 22257-1-AP (TSPY3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22257-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using TSPY3 Polyclonal antibody (22257-1-AP)

    IHC staining of human testis using 22257-1-AP (same clone as 22257-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis using 22257-1-AP (TSPY3 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 22257-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat testis tissue using TSPY3 Polyclonal antibody (22257-1-AP)

    IHC staining of rat testis using 22257-1-AP (same clone as 22257-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 22257-1-AP (TSPY3 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22257-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat testis tissue using TSPY3 Polyclonal antibody (22257-1-AP)

    IHC staining of rat testis using 22257-1-AP (same clone as 22257-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 22257-1-AP (TSPY3 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22257-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using TSPY3 Polyclonal antibody (22257-1-AP)

    IHC staining of human testis using 22257-1-AP (same clone as 22257-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis using 22257-1-AP (TSPY3 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 22257-1-PBS in a different storage buffer formulation.

TSPY3 Polyclonal antibody, PBS Only

TSPY3 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 22257-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

22257-1-PBS targets TSPY3 in WB, IHC, Indirect ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag17762

Product name: Recombinant human TSPY3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-308 aa of BC075016

Sequence: MRPEGSLTYRVPERLRQGFCGVGRAAQALVCASAKEGTAFRMEAVQEGAAGVESEQAALGEEAVLLLDDIMAEVEVVAEEEGLVERREEAQRAQQAVPGPGPMTPESALEELLAVQVELEPVNAQARKAFSRQREKMERRRKPQLDRRGAVIQSVPGFWANVIANHPQMSALITDEDEDMLSYMVSLEVEEEKHPVHLCKIMLFFRSNPYFQNKVITKEYLVNITEYRASHSTPIEWYPDYEVEAYRRRHHNSSLNFFNWFSDHNFAGSNKIAEILCKDLWRNPLQYYKRMKPPEEGTETSGDSQLLS

相同性解析による交差性が予測される生物種
Full Name testis specific protein, Y-linked 3
Calculated molecular weight 308 aa, 35 kDa
Observed molecular weight 39-40 kDa
GenBank accession numberBC075016
Gene Symbol TSPY3
Gene ID (NCBI) 728137
RRIDAB_11182707
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP0CV98
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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