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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TSPO/PBR Monoclonal antibody (68137-1-Ig)

    WB analysis using 68137-1-Ig (same clone as 68137-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68137-1-Ig (TSPO/PBR antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68137-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using TSPO/PBR Monoclonal antibody (68137-1-Ig)

    WB analysis using 68137-1-Ig (same clone as 68137-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68137-1-Ig (TSPO/PBR antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68137-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using TSPO/PBR Monoclonal antibody (68137-1-Ig)

    WB analysis of Jurkat using 68137-1-Ig (same clone as 68137-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 68137-1-Ig (TSPO/PBR antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68137-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of NIH/3T3 cells using TSPO/PBR Monoclonal antibody (68137-1-Ig)

    IF Staining of NIH/3T3 using 68137-1-Ig (same clone as 68137-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using TSPO/PBR antibody (68137-1-Ig, Clone: 1D1F12 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68137-1-PBS in a different storage buffer formulation.

TSPO Monoclonal antibody, PBS Only

TSPO Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 68137-1-PBS
Clone No.1D1F12

Host / Isotype

Mouse / IgM

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

TSPO/PBR, 1D1F12, BZRP, MBR, Peripheral-type benzodiazepine receptor

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68137-1-PBS targets TSPO in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgM
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag7475

Product name: Recombinant human TSPO protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-169 aa of BC001110

Sequence: MAPPWVPAMGFTLAPSLGCFVGSRFVHGEGLRWYAGLQKPSWHPPHWVLGPVWGTLYSAMGYGSYLVWKELGGFTEKAVVPLGLYTGQLALNWAWPPIFFGARQMGWALVDLLLVSGAAAATTVAWYQVSPLAARLLYPYLAWLAFATTLNYCVWRDNHGWHGGRRLPE

相同性解析による交差性が予測される生物種
Full Name translocator protein (18kDa)
Calculated molecular weight 18 kDa
Observed molecular weight18 kDa
GenBank accession numberBC001110
Gene Symbol TSPO
Gene ID (NCBI) 706
Conjugate Unconjugated
Form
FormLiquid
Purification MethodCaprylic acid/ammonium sulfate precipitation
UNIPROT IDP30536
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

TSPO encodes the translocator protein, belonging to tryptophan-rich sensory protein family. The protein is mainly localized in the outer mitochondrial membrane and expressed in steroid-synthesizing tissues (PMID:32881658, PMID:1326278). TSPO is involved in the translocation of cholesterol from the outer to inner mitochondrial membrane (PMID:21119734). In response to injury, TSPO has a upregulated expression in the peripheral nervous system (PMID:32423330, PMID:23251719). TSPO is also as a biomarker in a non-invasive procedure including detecting the inflammation in the heart and atherosclerotic plaques (PMID:24402571).

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