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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TSPO/PBR Monoclonal antibody (68137-1-Ig)

    WB analysis using 68137-1-Ig (same clone as 68137-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68137-1-Ig (TSPO/PBR antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68137-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using TSPO/PBR Monoclonal antibody (68137-1-Ig)

    WB analysis using 68137-1-Ig (same clone as 68137-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68137-1-Ig (TSPO/PBR antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68137-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using TSPO/PBR Monoclonal antibody (68137-1-Ig)

    WB analysis of Jurkat using 68137-1-Ig (same clone as 68137-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 68137-1-Ig (TSPO/PBR antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68137-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using TSPO/PBR Monoclonal antibody (68137-1-Ig)

    IF Staining of mouse brain using 68137-1-Ig (same clone as 68137-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using TSPO/PBR antibody (68137-1-Ig, Clone: 1D1F12 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68137-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of NIH/3T3 cells using TSPO/PBR Monoclonal antibody (68137-1-Ig)

    IF Staining of NIH/3T3 using 68137-1-Ig (same clone as 68137-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using TSPO/PBR antibody (68137-1-Ig, Clone: 1D1F12 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68137-1-PBS in a different storage buffer formulation.

TSPO/PBR Monoclonal antibody, PBS Only

TSPO/PBR Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 68137-1-PBS
Clone No.1D1F12

Host / Isotype

Mouse / IgM

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

TSPO, 1D1F12, BZRP, MBR, Peripheral-type benzodiazepine receptor

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68137-1-PBS targets TSPO/PBR in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgM
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag7475

Product name: Recombinant human TSPO protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-169 aa of BC001110

Sequence: MAPPWVPAMGFTLAPSLGCFVGSRFVHGEGLRWYAGLQKPSWHPPHWVLGPVWGTLYSAMGYGSYLVWKELGGFTEKAVVPLGLYTGQLALNWAWPPIFFGARQMGWALVDLLLVSGAAAATTVAWYQVSPLAARLLYPYLAWLAFATTLNYCVWRDNHGWHGGRRLPE

相同性解析による交差性が予測される生物種
Full Name translocator protein (18kDa)
Calculated molecular weight 18 kDa
Observed molecular weight18 kDa
GenBank accession numberBC001110
Gene Symbol TSPO
Gene ID (NCBI) 706
RRIDAB_2923664
Conjugate Unconjugated
Form
FormLiquid
Purification MethodCaprylic acid/ammonium sulfate precipitation
UNIPROT IDP30536
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

TSPO, also named Peripheral-type benzodiazepine receptor (PBR), encodes the translocator protein, belonging to tryptophan-rich sensory protein family. The protein is mainly localized in the outer mitochondrial membrane and expressed in steroid-synthesizing tissues (PMID:32881658, PMID:1326278). TSPO is involved in the translocation of cholesterol from the outer to inner mitochondrial membrane (PMID:21119734). In response to injury, TSPO has a upregulated expression in the peripheral nervous system (PMID:32423330, PMID:23251719). TSPO is also as a biomarker in a non-invasive procedure including detecting the inflammation in the heart and atherosclerotic plaques (PMID:24402571).

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