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Validation Data Gallery

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  • Western Blot (WB) analysis of K-562 cells using TSPAN7 Polyclonal antibody (18695-1-AP)

    WB analysis of K-562 using 18695-1-AP (same clone as 18695-1-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 18695-1-AP (TSPAN7 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18695-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of K-562 cells using TSPAN7 Polyclonal antibody (18695-1-AP)

    WB analysis of K-562 using 18695-1-AP (same clone as 18695-1-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 18695-1-AP (TSPAN7 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18695-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of K-562 cells using TSPAN7 Polyclonal antibody (18695-1-AP)

    WB analysis of K-562 using 18695-1-AP (same clone as 18695-1-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 18695-1-AP (TSPAN7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18695-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of K-562 cells using TSPAN7 Polyclonal antibody (18695-1-AP)

    IP experiment of K-562 using 18695-1-AP (same clone as 18695-1-PBS)

    IP result of anti-TSPAN7 (IP:18695-1-AP, 4ug; Detection:18695-1-AP 1:500) with K-562 cells lysate 3600ug. This data was developed using the same antibody clone with 18695-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human spleen tissue using TSPAN7 Polyclonal antibody (18695-1-AP)

    IHC staining of human spleen using 18695-1-AP (same clone as 18695-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human spleen using 18695-1-AP (TSPAN7 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 18695-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human spleen tissue using TSPAN7 Polyclonal antibody (18695-1-AP)

    IHC staining of human spleen using 18695-1-AP (same clone as 18695-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human spleen using 18695-1-AP (TSPAN7 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 18695-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse pancreas tissue using TSPAN7 Polyclonal antibody (18695-1-AP)

    IHC staining of mouse pancreas using 18695-1-AP (same clone as 18695-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 18695-1-AP (TSPAN7 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18695-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse pancreas tissue using TSPAN7 Polyclonal antibody (18695-1-AP)

    IHC staining of mouse pancreas using 18695-1-AP (same clone as 18695-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 18695-1-AP (TSPAN7 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18695-1-PBS in a different storage buffer formulation.

TSPAN7 Polyclonal antibody, PBS Only

TSPAN7 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 18695-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

TALLA-1, Tetraspanin-7, TALLA 1, MXS1, MRX58

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

18695-1-PBS targets TSPAN7 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

Peptide

相同性解析による交差性が予測される生物種
Full Name tetraspanin 7
Calculated molecular weight 28 kDa
Observed molecular weight 28-35 kDa
GenBank accession numberBC018036
Gene Symbol TSPAN7
Gene ID (NCBI) 7102
RRIDAB_2213450
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP41732
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

TSPAN7, also named as A15, MXS1, CD231, MRX58, CCG-B7, TM4SF2, TALLA-1, TM4SF2b and DXS1692E, is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. TSPAN7 is a cell surface glycoprotein and may have a role in the control of neurite outgrowth. TSPAN7 may be involved in cell proliferation and cell motility. It is not solely expressed in T cells, but also expressed in acute myelocytic leukemia cells of some patients. The antibody only recognize the protein TSPAN7, can't recognize the protein TSPAN6.

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