Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse colon tissue, rat colon tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 2 publications below |
Product Information
12293-1-AP targets TSPAN6 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2964 Product name: Recombinant human TSPAN6 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-245 aa of BC012389 Sequence: MASPSRRLQTKPVITCFKSVLLIYTFIFWITGVILLAVGIWGKVSLENYFSLLNEKATNVPFVLIATGTVIILLGTFGCFATCRASAWMLKLYAMFLTLVFLVELVAAIVGFVFRHEIKNSFKNNYEKALKQYNSTGDYRSHAVDKIQNTLHCCGVTDYRDWTDTNYYSEKGFPKSCCKLEDCTPQRDADKVNNEGCFIKVMTIIESEMGVVAGISFGVACFQLIGIFLAYCLSRAITNNQYEIV 相同性解析による交差性が予測される生物種 |
| Full Name | tetraspanin 6 |
| Calculated molecular weight | 245 aa, 28 kDa |
| Observed molecular weight | 25-30 kDa |
| GenBank accession number | BC012389 |
| Gene Symbol | TSPAN6 |
| Gene ID (NCBI) | 7105 |
| RRID | AB_2213446 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O43657 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
TSPAN6 (also known as TM4SF6) is a membrane protein of the tetraspanin superfamily, which are characterized by the presence of four conserved transmembrane regions. Tetraspanins have been involved in diverse processes such as cell activation and proliferation, adhesion and motility, differentiation, and cancer. TSPAN6 gene is widely expressed, with high mRNA levels in liver, pancreas, kidney, and ovary (PMID: 9782095). TSPAN6 has been reported as a negative regulator of the RLR pathway by interacting with MAVS in a ubiquitination-dependent manner (PMID: 22908223).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for TSPAN6 antibody 12293-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Stem Cell Res Ther Research on the TSPAN6 regulating the secretion of ADSCs-Exos through syntenin-1 and promoting wound healing | ||
Eur J Med Res Diagnostic and prognostic significance of tetraspanin 6 and its role in facilitating glioma progression |
