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Validation Data Gallery

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  • Western Blot (WB) analysis of human brain tissue using TSPAN33 Polyclonal antibody (14329-1-AP)

    WB analysis of human brain using 14329-1-AP (same clone as 14329-1-PBS)

    human brain tissue were subjected to SDS PAGE followed by western blot with 14329-1-AP (TSPAN33 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14329-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human plasma using TSPAN33 Polyclonal antibody (14329-1-AP)

    WB analysis of human plasma using 14329-1-AP (same clone as 14329-1-PBS)

    human blood were subjected to SDS PAGE followed by western blot with 14329-1-AP (TSPAN33 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14329-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human hepatocirrhosis tissue using TSPAN33 Polyclonal antibody (14329-1-AP)

    IHC staining of human hepatocirrhosis using 14329-1-AP (same clone as 14329-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 14329-1-AP (TSPAN33 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 14329-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human hepatocirrhosis tissue using TSPAN33 Polyclonal antibody (14329-1-AP)

    IHC staining of human hepatocirrhosis using 14329-1-AP (same clone as 14329-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 14329-1-AP (TSPAN33 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 14329-1-PBS in a different storage buffer formulation.

TSPAN33 Polyclonal antibody, PBS Only

TSPAN33 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 14329-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

14329-1-PBS targets TSPAN33 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag5597

Product name: Recombinant human TSPAN33 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 116-236 aa of BC044244

Sequence: FVFSDKARGKVSEIINNAIVHYRDDLDLQNLIDFGQKKFSCCGGISYKDWSQNMYFNCSEDNPSRERCSVPYSCCLPTPDQAVINTMCGQGMQAFDYLEASKVIYTNGCIDKLVNWIHSNL

相同性解析による交差性が予測される生物種
Full Name tetraspanin 33
Calculated molecular weight 32 kDa
Observed molecular weight 64-72 kDa
GenBank accession numberBC044244
Gene Symbol TSPAN33
Gene ID (NCBI) 340348
RRIDAB_10644331
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ86UF1
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

TSPAN33 is a membrane protein of the tetraspanin superfamily, which are characterized by the presence of four conserved transmembrane regions. Tetraspanins have been involved in diverse processes such as cell activation and proliferation, adhesion and motility, differentiation, and cancer. TSPAN33 belongs to the TSPANC8 subfamily, which also include TSPAN5, TSPAN10, TSPAN14, TSPAN15, and TSPAN17. It has been reported that TSPANC8 tetraspanins interact with ADAM10 and have a conserved function in the regulation of ADAM10 trafficking and activity, thereby positively regulating Notch receptor activation (PMID: 23091066; 23035126).

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