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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TSPAN1 Polyclonal antibody (16058-1-AP)

    WB analysis using 16058-1-AP (same clone as 16058-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 16058-1-AP (TSPAN1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16058-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using TSPAN1 Polyclonal antibody (16058-1-AP)

    WB analysis of HeLa using 16058-1-AP (same clone as 16058-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 16058-1-AP (TSPAN1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16058-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of COLO 320 cells using TSPAN1 Polyclonal antibody (16058-1-AP)

    WB analysis of COLO 320 using 16058-1-AP (same clone as 16058-1-PBS)

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 16058-1-AP (TSPAN1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16058-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat colon tissue using TSPAN1 Polyclonal antibody (16058-1-AP)

    WB analysis of rat colon using 16058-1-AP (same clone as 16058-1-PBS)

    rat colon tissue were subjected to SDS PAGE followed by western blot with 16058-1-AP (TSPAN1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16058-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using TSPAN1 Polyclonal antibody (16058-1-AP)

    IHC staining of human colon using 16058-1-AP (same clone as 16058-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 16058-1-AP (TSPAN1 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 16058-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using TSPAN1 Polyclonal antibody (16058-1-AP)

    IHC staining of human colon using 16058-1-AP (same clone as 16058-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 16058-1-AP (TSPAN1 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 16058-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

TSPAN1 Polyclonal antibody, PBS Only

TSPAN1 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 16058-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

16058-1-PBS targets TSPAN1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag8786

Product name: Recombinant human TSPAN1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-215 aa of BC013404

Sequence: MQCFSFIKTMMILFNLLIFLCGAALLAVGIWVSIDGASFLKIFGPLSSSAMQFVNVGYFLIAAGVVVFALGFLGCYGAKTESKCALVTFFFILLLIFIAEVAAAVVALVYTTMAEHFLTLLVVPAIKKDYGSQEDFTQVWNTTMKGLKCCGFTNYTDFEDSPYFKENSAFPPFCCNDNVTNTANETCTKQKAHDQKVEGCFNQLLYDIRTNAVTV

相同性解析による交差性が予測される生物種
Full Name tetraspanin 1
Calculated molecular weight 241 aa, 26 kDa
Observed molecular weight 26-30 kDa
GenBank accession numberBC013404
Gene Symbol TSPAN1
Gene ID (NCBI) 10103
RRIDAB_2878210
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDO60635
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

TSPAN1 is a membrane protein of the tetraspanin superfamily, which is characterized by the presence of four conserved transmembrane regions. Tetraspanins have been involved in diverse processes such as cell activation and proliferation, adhesion and motility, differentiation, and cancer. Altered TSPAN1 expression has been associated with the progression of some neoplasms including hepatocellular carcinoma, colorectal, gastric, and breast cancers (PMID: 20890423; 19437569; 18822690; 20680643).

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