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Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human pituitary tissue using TSH Beta Monoclonal antibody (66750-1-Ig)

    IHC staining of human pituitary using 66750-1-Ig (same clone as 66750-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pituitary tissue slide using 66750-1-Ig (TSH beta antibody) at dilution of 1:6000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66750-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pituitary tissue using TSH Beta Monoclonal antibody (66750-1-Ig)

    IHC staining of human pituitary using 66750-1-Ig (same clone as 66750-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pituitary tissue slide using 66750-1-Ig (TSH beta antibody) at dilution of 1:6000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66750-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human pituitary tissue using TSH Beta Monoclonal antibody (66750-1-Ig)

    IF Staining of human pituitary using 66750-1-Ig (same clone as 66750-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human pituitary tissue using TSH Beta antibody (66750-1-Ig, Clone: 2G1H3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66750-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human pituitary tissue using TSH Beta Monoclonal antibody (66750-1-Ig)

    IF Staining of human pituitary using 66750-1-Ig (same clone as 66750-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human pituitary tissue using TSH Beta antibody (66750-1-Ig, Clone: 2G1H3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66750-1-PBS in a different storage buffer formulation.

TSH Beta Monoclonal antibody, PBS Only

TSH Beta Monoclonal Antibody for IHC, IF-P, Indirect ELISA
Cat No. 66750-1-PBS
Clone No.2G1H3

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

IHC, IF-P, Indirect ELISA

Synonyms

CHNG4, Thyrotropin alfa, Thyrotropin beta chain, Thyrotropin subunit beta, TSH B, TSH BETA, TSHB

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66750-1-PBS targets TSH Beta in IHC, IF-P, Indirect ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag27153

Product name: Recombinant human TSHB protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 65-138 aa of BC069298

Sequence: YALSQDVCTYRDFIYRTVEIPGCPLHVAPYFSYPVALSCKCGKCNTDYSDCIHEAIKTNYCTKPQKSYLVGFSV

相同性解析による交差性が予測される生物種
Full Name thyroid stimulating hormone, beta
Calculated molecular weight 138 aa, 16 kDa
GenBank accession numberBC069298
Gene Symbol TSH beta
Gene ID (NCBI) 7252
RRIDAB_2882097
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP01222
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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