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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Multi-rAb™ TSG101 Multi-Recombinant antibody (RMX00026)

    WB analysis using RMX00026

    Various lysates were subjected to SDS PAGE followed by western blot with RMX00026 (TSG101 antibody) at dilution of 1:12000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using Multi-rAb™ TSG101 Multi-Recombinant antibody (RMX00026)

    IP experiment of HeLa using RMX00026

    IP result of anti-TSG101 (IP:RMX00026, 4ug; Detection:RMX00026 1:10000) with HeLa cells lysate 1480 ug.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using Multi-rAb™ TSG101 Multi-Recombinant antibody (RMX00026)

    IHC staining of human colon cancer using RMX00026

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using RMX00026 (TSG101 antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Multi-rAb™ TSG101 Multi-Recombinant antibody (RMX00026)

    IF Staining of HeLa using RMX00026

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using TSG101 antibody (RMX00026) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Multi-rAb™ TSG101 Multi-Recombinant antibody

TSG101 Multi-Recombinant Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. RMX00026

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

ESCRT-I complex subunit TSG101, TSG 10, TSG 101, TSG10, VPS 23

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, Jurkat cells, K-562 cells, NIH/3T3 cells, HSC-T6 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:300-1:1200
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

RMX00026 targets TSG101 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Multi-Recombinant
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name tumor susceptibility gene 101
Calculated molecular weight 44 kDa
Observed molecular weight 44 kDa
GenBank accession numberBC002487
Gene Symbol TSG101
Gene ID (NCBI) 7251
Conjugate Unconjugated
Form Liquid
Purification MethodN/A
UNIPROT IDQ99816
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TSG101(Tumor susceptibility gene 101 protein) is essential for endosomal sorting, membrane receptor degradation and the final stages of cytokinesis. It plays a crucial role for cell proliferation and cell survival. TSG101 has been identified as a candidate tumor suppressor gene and belongs to the ubiquitin-conjugating enzyme family. TSG101 is a marker for exosome. This protein has 2 isoforms produced by alternative splicing with the molecular mass of 44 and 32 kDa.

Protocols

Product Specific Protocols
WB protocol for TSG101 antibody RMX00026Download protocol
IHC protocol for TSG101 antibody RMX00026Download protocol
IF protocol for TSG101 antibody RMX00026Download protocol
IP protocol for TSG101 antibody RMX00026Download protocol
Standard Protocols
Click here to view our Standard Protocols