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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TSFM Polyclonal antibody

TSFM Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11701-1-AP

Synonyms

COXPD3, EF TS, EF Tsmt, TSFM

Validation Data Gallery

Applications:
  • WB analysis of HeLa using 11701-1-AP

    WB analysis of HeLa using 11701-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 11701-1-AP (TSFM Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 11701-1-AP

    WB analysis of HEK-293 using 11701-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 11701-1-AP (TSFM Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis of PC-3 using 11701-1-AP

    WB analysis of PC-3 using 11701-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 11701-1-AP (TSFM Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 11701-1-AP

    IHC staining of human colon cancer using 11701-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11701-1-AP (TSFM antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human colon cancer using 11701-1-AP

    IHC staining of human colon cancer using 11701-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11701-1-AP (TSFM antibody) at dilution of 1:200 (under 40x lens).

  • IHC staining of human kidney using 11701-1-AP

    IHC staining of human kidney using 11701-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 11701-1-AP (TSFM antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human kidney using 11701-1-AP

    IHC staining of human kidney using 11701-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 11701-1-AP (TSFM antibody) at dilution of 1:200 (under 40x lens).

  • IHC staining of human stomach cancer using 11701-1-AP

    IHC staining of human stomach cancer using 11701-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 11701-1-AP (TSFM antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human stomach cancer using 11701-1-AP

    IHC staining of human stomach cancer using 11701-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 11701-1-AP (TSFM antibody) at dilution of 1:200 (under 40x lens).

  • IF Staining of HeLa using 11701-1-AP

    IF Staining of HeLa using 11701-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 11701-1-AP (TSFM antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHeLa cells, PC-3 cells, HEK-293 cells
Positive IHC detected inhuman colon cancer tissue, human kidney tissue, human stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

11701-1-AP targets TSFM in WB, IF, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TSFM fusion protein Ag2308 相同性解析による交差性が予測される生物種
Full Name Ts translation elongation factor, mitochondrial
Calculated molecular weight 35 kDa
Observed molecular weight 35 kDa
GenBank accession numberBC022862
Gene symbol TSFM
Gene ID (NCBI) 10102
RRIDAB_2207953
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for TSFM antibody 11701-1-APDownload protocol
IHC protocol for TSFM antibody 11701-1-APDownload protocol
IF protocol for TSFM antibody 11701-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols