Validation Data Gallery

Filter: AllWBIHCIF/ICC

WB analysis of HeLa using 11701-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 11701-1-AP (TSFM Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 11701-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 11701-1-AP (TSFM Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of PC-3 using 11701-1-AP
PC-3 cells were subjected to SDS PAGE followed by western blot with 11701-1-AP (TSFM Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
IHC staining of human colon cancer using 11701-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11701-1-AP (TSFM antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human colon cancer using 11701-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11701-1-AP (TSFM antibody) at dilution of 1:200 (under 40x lens).
IHC staining of human stomach cancer using 11701-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 11701-1-AP (TSFM antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human stomach cancer using 11701-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 11701-1-AP (TSFM antibody) at dilution of 1:200 (under 40x lens).
IHC staining of human kidney using 11701-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 11701-1-AP (TSFM antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human kidney using 11701-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 11701-1-AP (TSFM antibody) at dilution of 1:200 (under 40x lens).
IF Staining of HeLa using 11701-1-AP
Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 11701-1-AP (TSFM antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

Featured Product
KD/KO Validated

TSFM Polyclonal antibody

TSFM Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 11701-1-AP

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

EF-TsMt, EF-Ts, EF Tsmt, EF TS, COXPD3

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (2)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HeLa cells, PC-3 cells, HEK-293 cells
Positive IHC detected in	human colon cancer tissue, human kidney tissue, human stomach cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 2 publications below

Product Information

11701-1-AP targets TSFM in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag2308 Product name: Recombinant human TSFM protein Source: e coli.-derived, T-GST Tag: GST Domain: 1-346 aa of BC022862 Sequence: MSLLRSLRVFLVARTGSYPAGSLLRQSPQPRHTFYAGPRLSASASSKELLMKLRRKTGYSFVNCKKALETCGGDLKQAEIWLHKEAQKEGWSKAAKLQGRKTKEGLIGLLQEGNTTVLVEVNCETDFVSRNLKFQLLVQQVALGTMMHCQTLKDQPSAYSKVQWLTPVNLALWEAEAGGSLEGFLNSSELSGLPAGPDREGSLKDQLALAIGKLGENMILKRAAWVKVPSGFYVGSYVHGAMQSPSLHKLVLGKYGALVICETSEQKTNLEDVGRRLGQHVVGMAPLSVGSLDDEPGGEAETKMLSQPYLLDPSITLGQYVQPQGVSVVDFVRFECGEGEEAAETE 相同性解析による交差性が予測される生物種
Full Name	Ts translation elongation factor, mitochondrial
Calculated molecular weight	35 kDa
Observed molecular weight	35 kDa
GenBank accession number	BC022862
Gene Symbol	TSFM
Gene ID (NCBI)	10102
RRID	AB_2207953
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P43897
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for TSFM antibody 11701-1-AP	Download protocol
IHC protocol for TSFM antibody 11701-1-AP	Download protocol
IF protocol for TSFM antibody 11701-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Mol Biol Rep Short-term regulation of TSFM level does not alter amyloidogenesis and mitochondrial function in type-specific cells Authors - Xiao-Yun Li KD Validated View Article
human	WB	Cell Death Dis piR-26441 inhibits mitochondrial oxidative phosphorylation and tumorigenesis in ovarian cancer through m6A modification by interacting with YTHDC1 Authors - Jing Yuan View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of HeLa using 11701-1-AP

WB analysis of HEK-293 using 11701-1-AP

WB analysis of PC-3 using 11701-1-AP

IHC staining of human colon cancer using 11701-1-AP