Validation Data Gallery
Tested Applications
| Positive WB detected in | Jurkat cells, MCF-7 cells, PC-3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
14320-1-AP targets TSEN54 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag5561 Product name: Recombinant human TSEN54 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 178-526 aa of BC047793 Sequence: SPYERQLNLDASVQHLEDGDGKRKRSSSSPRSINKKAKALDNSLQPKSLAASSPPPCSQPSQCPEEKPQESSPMKGPGGPFQLLGSLGPSPGPAREGVGCSWESGRAENGVTGAGKRRWNFEQISFPNMASDSRHTLLRAPAPELLPANVAGRETDAESWCQKLNQRKENLSRREREHHAEAAQFQEDVNADPEVQRCSSWREYKELLQRRQVQRSQRRAPHLWGQPVTPLLSPGQASSPAVVLQHISVLQTTHLPDGGVRLLEKSGGLEIIFDVYQADAVATFRKNNPGKPYARMCISGFDEPVPDLCSLKRLSYQSGDVPLIFALVDHGDISFYSFRDFTLPQDVGH 相同性解析による交差性が予測される生物種 |
| Full Name | tRNA splicing endonuclease 54 homolog (S. cerevisiae) |
| Calculated molecular weight | 59 kDa |
| Observed molecular weight | 54 kDa |
| GenBank accession number | BC047793 |
| Gene Symbol | TSEN54 |
| Gene ID (NCBI) | 283989 |
| RRID | AB_3669184 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q7Z6J9 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
TSEN54 (tRNA-splicing endonuclease subunit Sen54) encodes a subunit of the tRNA-splicing endonuclease complex, which catalyzes the identification and cleavage of introns from precursor tRNAs (PMID: 28083540).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for TSEN54 antibody 14320-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |