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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TSEN54 Polyclonal antibody (14320-1-AP)

    WB analysis using 14320-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14320-1-AP (TSEN54 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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TSEN54 Polyclonal antibody

TSEN54 Polyclonal Antibody for WB, ELISA
Cat No. 14320-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

SEN54L, SEN54 homolog, SEN54, PCH4, PCH2A

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inJurkat cells, MCF-7 cells, PC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14320-1-AP targets TSEN54 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TSEN54 fusion protein Ag5561 相同性解析による交差性が予測される生物種
Full Name tRNA splicing endonuclease 54 homolog (S. cerevisiae)
Calculated molecular weight 59 kDa
Observed molecular weight54 kDa
GenBank accession numberBC047793
Gene Symbol TSEN54
Gene ID (NCBI) 283989
RRIDAB_3669184
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ7Z6J9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TSEN54 (tRNA-splicing endonuclease subunit Sen54) encodes a subunit of the tRNA-splicing endonuclease complex, which catalyzes the identification and cleavage of introns from precursor tRNAs (PMID: 28083540).

Protocols

Product Specific Protocols
WB protocol for TSEN54 antibody 14320-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols