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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TSEN34 Recombinant antibody (84102-6-RR)

    WB analysis using 84102-6-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84102-6-RR (TSEN34 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using TSEN34 Recombinant antibody (84102-6-RR)

    IF Staining of MCF-7 using 84102-6-RR

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using TSEN34 antibody (84102-6-RR, Clone: 241155D2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

TSEN34 Recombinant monoclonal antibody

TSEN34 Uni-rAb® Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 84102-6-RR
Clone No.241155D2

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

SEN34, PCH2C, LENG5, HsSen34, EC:4.6.1.16

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHEK-293 cells, A431 cells, A549 cells, HeLa cells, MCF-7 cells, HCT 116 cells
Positive IF/ICC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84102-6-RR targets TSEN34 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag35120

Product name: Recombinant human TSEN34 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 52-165 aa of BC020805

Sequence: LMPEEARLLAEIGAVTLVSAPRPDSRHHSLALTSFKRQQEESFQEQSALAAEARETRRQELLEKITEGQAAKKQKLEQASGASSSQEAGSSQAAKEDETSDGQASGEQEEAGPS

相同性解析による交差性が予測される生物種
Full Name tRNA splicing endonuclease 34 homolog (S. cerevisiae)
Calculated molecular weight 34 kDa
Observed molecular weight34 kDa
GenBank accession numberBC020805
Gene Symbol TSEN34
Gene ID (NCBI) 79042
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purfication
UNIPROT IDQ9BSV6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The human tRNA splicing endonuclease consists of a heterotetramer complex formed by the four TSEN proteins, which were identified by homology with their yeast counterparts. TSEN2 and TSEN34 are the catalytic subunits, which form a compound active site, whereas TSEN54 and TSEN15 are structural proteins. (PMID: 27392077)

Protocols

Product Specific Protocols
IF protocol for TSEN34 antibody 84102-6-RRDownload protocol
WB protocol for TSEN34 antibody 84102-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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