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Validation Data Gallery

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  • Western Blot (WB) analysis of MCF-7 cells using TSEN34 Recombinant antibody (84102-5-RR)

    WB analysis of MCF-7 using 84102-5-RR

    WB result of TSEN34 antibody (84102-5-RR; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TSEN34 transfected MCF-7 cells.

  • Western Blot (WB) analysis of various lysates using TSEN34 Recombinant antibody (84102-5-RR)

    WB analysis using 84102-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84102-5-RR (TSEN34 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using TSEN34 Recombinant antibody (84102-5-RR)

    IF Staining of MCF-7 using 84102-5-RR

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using TSEN34 antibody (84102-5-RR, Clone: 241155G4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of HeLa cells using TSEN34 Recombinant antibody (84102-5-RR)

    FC experiment of HeLa using 84102-5-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug TSEN34 Recombinant antibody (84102-5-RR, Clone:241155G4) and Multi-rAb CoraLite ® Plus 647-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR005)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with 0.1% TritonX-100.

  • Affinity and Kinetic Characterization of 84102-5-RR

    Affinity and Kinetic Characterization of 84102-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84102-5-RR against Human TSEN34 were performed. The affinity constant is 4.01 nM.

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  • KD/KO Validated

TSEN34 Recombinant antibody

TSEN34 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 84102-5-RR
Clone No.241155G4

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), ELISA

Synonyms

241155G4, EC:4.6.1.16, HsSen34, LENG5, PCH2C

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHEK-293 cells, MCF-7 cells, A431 cells, A549 cells, HeLa cells, HCT 116 cells, U2OS cells
Positive IF/ICC detected inMCF-7 cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84102-5-RR targets TSEN34 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen TSEN34 fusion protein Ag35120 相同性解析による交差性が予測される生物種
Full Name tRNA splicing endonuclease 34 homolog (S. cerevisiae)
Calculated molecular weight 34 kDa
Observed molecular weight34-36 kDa
GenBank accession numberBC020805
Gene Symbol TSEN34
Gene ID (NCBI) 79042
RRIDAB_3671667
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ9BSV6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The human tRNA splicing endonuclease consists of a heterotetramer complex formed by the four TSEN proteins, which were identified by homology with their yeast counterparts. TSEN2 and TSEN34 are the catalytic subunits, which form a compound active site, whereas TSEN54 and TSEN15 are structural proteins. (PMID: 27392077)

Protocols

Product Specific Protocols
WB protocol for TSEN34 antibody 84102-5-RRDownload protocol
IF protocol for TSEN34 antibody 84102-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols