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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TSEN34 Polyclonal antibody (31212-1-AP)

    WB analysis using 31212-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31212-1-AP (TSEN34 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using TSEN34 Polyclonal antibody (31212-1-AP)

    WB analysis using 31212-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31212-1-AP (TSEN34 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using TSEN34 Polyclonal antibody (31212-1-AP)

    WB analysis of MCF-7 using 31212-1-AP

    WB result of TSEN34 antibody (31212-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TSEN34 transfected MCF-7 cells.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using TSEN34 Polyclonal antibody (31212-1-AP)

    IF Staining of MCF-7 using 31212-1-AP

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using TSEN34 antibody (31212-1-AP) at dilution of 1:600 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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  • KD/KO Validated

TSEN34 Polyclonal antibody

TSEN34 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 31212-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

EC:4.6.1.16, HsSen34, LENG5, PCH2C, SEN34

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHCT 116 cells, HEK-293 cells, MCF-7 cells, HeLa cells, HEK-293T cells
Positive IF/ICC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunofluorescence (IF)/ICCIF/ICC : 1:300-1:1200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

31212-1-AP targets TSEN34 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TSEN34 fusion protein Ag35120 相同性解析による交差性が予測される生物種
Full Name tRNA splicing endonuclease 34 homolog (S. cerevisiae)
Calculated molecular weight 34 kDa
Observed molecular weight34-36 kDa
GenBank accession numberBC020805
Gene Symbol TSEN34
Gene ID (NCBI) 79042
RRIDAB_3669904
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ9BSV6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The human tRNA splicing endonuclease consists of a heterotetramer complex formed by the four TSEN proteins, which were identified by homology with their yeast counterparts. TSEN2 and TSEN34 are the catalytic subunits, which form a compound active site, whereas TSEN54 and TSEN15 are structural proteins. (PMID: 27392077)

Protocols

Product Specific Protocols
WB protocol for TSEN34 antibody 31212-1-APDownload protocol
IF protocol for TSEN34 antibody 31212-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols