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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TSC22D2 Polyclonal antibody (25418-1-AP)

    WB analysis using 25418-1-AP (same clone as 25418-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 25418-1-AP (TSC22D2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25418-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MDA-MB-453s cells using TSC22D2 Polyclonal antibody (25418-1-AP)

    WB analysis of MDA-MB-453s using 25418-1-AP (same clone as 25418-1-PBS)

    MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 25418-1-AP (TSC22D2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25418-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using TSC22D2 Polyclonal antibody (25418-1-AP)

    WB analysis of HepG2 using 25418-1-AP (same clone as 25418-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 25418-1-AP (TSC22D2 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night. This data was developed using the same antibody clone with 25418-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HepG2 cells using TSC22D2 Polyclonal antibody (25418-1-AP)

    IP experiment of HepG2 using 25418-1-AP (same clone as 25418-1-PBS)

    IP result of anti-TSC22D2 (IP:25418-1-AP, 5ug; Detection:25418-1-AP 1:300) with HepG2 cells lysate 2500ug. This data was developed using the same antibody clone with 25418-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using TSC22D2 Polyclonal antibody (25418-1-AP)

    IHC staining of human kidney using 25418-1-AP (same clone as 25418-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25418-1-AP (TSC22D2 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 25418-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using TSC22D2 Polyclonal antibody (25418-1-AP)

    IHC staining of human colon using 25418-1-AP (same clone as 25418-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 25418-1-AP (TSC22D2 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 25418-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using TSC22D2 Polyclonal antibody (25418-1-AP)

    IHC staining of human kidney using 25418-1-AP (same clone as 25418-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25418-1-AP (TSC22D2 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 25418-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using TSC22D2 Polyclonal antibody (25418-1-AP)

    IHC staining of human colon using 25418-1-AP (same clone as 25418-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 25418-1-AP (TSC22D2 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 25418-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using TSC22D2 Polyclonal antibody (25418-1-AP)

    IF Staining of HepG2 using 25418-1-AP (same clone as 25418-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using TSC22D2 antibody (25418-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 25418-1-PBS in a different storage buffer formulation.

TSC22D2 Polyclonal antibody, PBS Only

TSC22D2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 25418-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

TSC22 domain family protein 2, TILZ4c, TILZ4b, TILZ4a, TILZ4

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

25418-1-PBS targets TSC22D2 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag22294

Product name: Recombinant human TSC22D2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 190-543 aa of BC044643

Sequence: SDSSVLTRSGDCIRHSSTFDQTAERDSGLGATGGSVVVVVASMQGAHGPESGTDSSLTAVSQLPPSEKMSQPTPAQPQSFSVGQPQPPPPPVGGAVAQSSAPLPPFPGAATGPQPMMAAAQPSQPQGAGPGGQTLPPTNVTLAQPAMSLPPQPGPAVGAPAAQQPQQFAYPQPQIPPGHLLPVQPSGQSEYLQQHVAGLQPPSPAQPSSTGAAASPATAATLPVGTGQNASSVGAQLMGASSQPSEAMAPRTGPAQGGQVAPCQPTGVPPATVGGVVQPCLGPAGAGQPQSVPPPQMGGSGPLSAVPGGPHAVVPGVPNVPAAVPAPSVPSVSTTSVTMPNVPAPLAQSQQLSS

相同性解析による交差性が予測される生物種
Full Name TSC22 domain family, member 2
Calculated molecular weight 780 aa, 79 kDa
Observed molecular weight 100-110 kDa
GenBank accession numberBC044643
Gene Symbol TSC22D2
Gene ID (NCBI) 9819
RRIDAB_2880069
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDO75157
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

TSC22D2 (transforming growth factor β-stimulated clone 22 domain family, member 2) is a member of the TSC-22 domain family comprising putative transcription factors that are characterized by a carboxy-terminal leucine zipper and an adjacent TSC-box. TSC22D2 is expressed at low levels in CRC and that TSC22D2 overexpression significantly inhibited the growth rate of cancer cells (PMID: 27573352).

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