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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Hamartin/TSC1 Polyclonal antibody

Hamartin/TSC1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 29906-1-AP

Synonyms

Hamartin, KIAA0243, LAM, TSC, TSC1, tuberous sclerosis 1, Tuberous sclerosis 1 protein

Validation Data Gallery

Applications:
  • WB analysis of mouse kidney using 29906-1-AP

    WB analysis of mouse kidney using 29906-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 29906-1-AP (Hamartin/TSC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis using 29906-1-AP

    WB analysis using 29906-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29906-1-AP (TSC1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse kidney using 29906-1-AP

    IHC staining of mouse kidney using 29906-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 29906-1-AP (Hamartin/TSC1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse kidney using 29906-1-AP

    IHC staining of mouse kidney using 29906-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 29906-1-AP (Hamartin/TSC1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse skeletal muscle using 29906-1-AP

    IHC staining of mouse skeletal muscle using 29906-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 29906-1-AP (Hamartin/TSC1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse skeletal muscle using 29906-1-AP

    IHC staining of mouse skeletal muscle using 29906-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 29906-1-AP (Hamartin/TSC1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inHeLa cells, mouse kidney tissue, MCF-7 cells
Positive IHC detected inmouse kidney tissue, mouse skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

29906-1-AP targets Hamartin/TSC1 in WB, IHC, ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Hamartin/TSC1 fusion protein Ag31621 相同性解析による交差性が予測される生物種
Full Name tuberous sclerosis 1
Calculated molecular weight130KD
Observed molecular weight140-145 kDa
GenBank accession numberNM_000368
Gene symbol TSC1
Gene ID (NCBI) 7248
RRIDAB_2918352
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

TSC1, also named as hamartin, interacts with TSC2 to form a protein complex that inhibits the nutrient-mediated or growth factor-stimulated phosphorylation of S6K1 and EIF4EBP1 by negatively regulating mTORC1 signaling (PubMed: 12271141, PubMed: 28215400). It can recruit TSC2 to HSP90AA1 and stabilizes TSC2 by preventing the interaction between TSC2 and ubiquitin ligase HERC1 (PubMed: 16464865, PubMed: 29127155). The loss of TSC1/TSC2 in mouse neurons resulted in a block in oligodendrocyte hypomyelination in vivo (PubMed: 28183733).

Protocols

Product Specific Protocols
WB protocol for Hamartin/TSC1 antibody 29906-1-APDownload protocol
IHC protocol for Hamartin/TSC1 antibody 29906-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Elife

A cleaved METTL3 potentiates the METTL3-WTAP interaction and breast cancer progression

Authors - Chaojun Yan
View Article