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TRUB2 Polyclonal antibody
TRUB2 Polyclonal Antibody for IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, IHC, ELISA
Conjugate
Unconjugated
Cat no : 19891-1-AP
Synonyms
Validation Data Gallery
Applications:
Tested Applications
| Positive WB detected in | HT-1080 cells, HeLa cells, HepG2 cells, K-562 cells |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy TRUB2 Ready-To-Use IHC Kit. TRUB2 primary antibody included.
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| WB | See 2 publications below |
Product Information
19891-1-AP targets TRUB2 in WB, IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | TRUB2 fusion protein Ag13726 相同性解析による交差性が予測される生物種 |
| Full Name | TruB pseudouridine (psi) synthase homolog 2 (E. coli) |
| Calculated molecular weight | 331 aa, 37 kDa |
| Observed molecular weight | 37 kDa |
| GenBank accession number | BC001457 |
| Gene symbol | TRUB2 |
| Gene ID (NCBI) | 26995 |
| RRID | AB_10640900 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for TRUB2 antibody 19891-1-AP | Download protocol |
| IHC protocol for TRUB2 antibody 19891-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
EMBO Rep A pseudouridine synthase module is essential for mitochondrial protein synthesis and cell viability. | ||
Nat Cell Biol Regulators of mitonuclear balance link mitochondrial metabolism to mtDNA expression |
