Validation Data Gallery
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Tested Applications
| Positive WB detected in | HEK-293T cells, HepG2 cells |
| Positive IHC detected in | mouse kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
RMX00073 targets TRUB1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Multi-Recombinant |
| Type | Antibody |
| Immunogen |
Recombinant protein 相同性解析による交差性が予測される生物種 |
| Full Name | TruB pseudouridine (psi) synthase homolog 1 (E. coli) |
| Calculated molecular weight | 349 aa, 37 kDa |
| Observed molecular weight | 37 kDa, 40 kDa |
| GenBank accession number | BC030601 |
| Gene Symbol | TRUB1 |
| Gene ID (NCBI) | 142940 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | N/A |
| UNIPROT ID | Q8WWH5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}} |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
TRUB1 gene encodes a 349-amino acid product, with a VFAVHKPKGPTSA box in positions 71-83 corresponding to motif I of the TruB family (probably involved in conserving protein structure). This protein may be responsible for synthesis of pseudouridine from uracil in transfer RNAs.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for TRUB1 antibody RMX00073 | Download protocol |
| WB protocol for TRUB1 antibody RMX00073 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |