Validation Data Gallery
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Tested Applications
| Positive WB detected in | HEK-293T cells, HepG2 cells |
| Positive IP detected in | mouse liver tissue |
| Positive IHC detected in | human kidney tissue, mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 2 publications below |
| IF | See 1 publications below |
Product Information
12520-1-AP targets TRUB1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | TRUB1 fusion protein Ag3213 相同性解析による交差性が予測される生物種 |
| Full Name | TruB pseudouridine (psi) synthase homolog 1 (E. coli) |
| Calculated molecular weight | 349 aa, 37 kDa |
| Observed molecular weight | 37 kDa |
| GenBank accession number | BC030601 |
| Gene Symbol | TRUB1 |
| Gene ID (NCBI) | 142940 |
| RRID | AB_10640437 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8WWH5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
TRUB1 gene encodes a 349-amino acid product, with a VFAVHKPKGPTSA box in positions 71-83 corresponding to motif I of the TruB family (probably involved in conserving protein structure). This protein may be responsible for synthesis of pseudouridine from uracil in transfer RNAs.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for TRUB1 antibody 12520-1-AP | Download protocol |
| IHC protocol for TRUB1 antibody 12520-1-AP | Download protocol |
| IP protocol for TRUB1 antibody 12520-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Methods Absolute quantitative and base-resolution sequencing reveals comprehensive landscape of pseudouridine across the human transcriptome | ||
bioRxiv Probing enzyme-dependent pseudouridylation using direct RNA sequencing to assess neuronal epitranscriptome plasticity | ||
Nucleic Acids Res A bisulfite-assisted and ligation-based qPCR amplification technology for locus-specific pseudouridine detection at base resolution
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