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Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human liver cancer tissue using TRPM8 Polyclonal antibody (12813-1-AP)

    IHC staining of human liver cancer using 12813-1-AP (same clone as 12813-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12813-1-AP (TRPM8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12813-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using TRPM8 Polyclonal antibody (12813-1-AP)

    IHC staining of human liver cancer using 12813-1-AP (same clone as 12813-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12813-1-AP (TRPM8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12813-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using TRPM8 Polyclonal antibody (12813-1-AP)

    IHC staining of human liver cancer using 12813-1-AP (same clone as 12813-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12813-1-AP (TRPM8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12813-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using TRPM8 Polyclonal antibody (12813-1-AP)

    IHC staining of human liver cancer using 12813-1-AP (same clone as 12813-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12813-1-AP (TRPM8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12813-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using TRPM8 Polyclonal antibody (12813-1-AP)

    IHC staining of human prostate cancer using 12813-1-AP (same clone as 12813-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12813-1-AP (TRPM8 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12813-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using TRPM8 Polyclonal antibody (12813-1-AP)

    IHC staining of human prostate cancer using 12813-1-AP (same clone as 12813-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12813-1-AP (TRPM8 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12813-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using TRPM8 Polyclonal antibody (12813-1-AP)

    IHC staining of human colon cancer using 12813-1-AP (same clone as 12813-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12813-1-AP (TRPM8 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12813-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using TRPM8 Polyclonal antibody (12813-1-AP)

    IHC staining of human colon cancer using 12813-1-AP (same clone as 12813-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12813-1-AP (TRPM8 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12813-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of SH-SY5Y cells using TRPM8 Polyclonal antibody (12813-1-AP)

    IF Staining of SH-SY5Y using 12813-1-AP (same clone as 12813-1-PBS)

    Immunofluorescent analysis of SH-SY5Y cells using 12813-1-AP (TRPM8 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG. This data was developed using the same antibody clone with 12813-1-PBS in a different storage buffer formulation.

TRPM8 Polyclonal antibody, PBS Only

TRPM8 Polyclonal Antibody for IHC, IF/ICC, Indirect ELISA
Cat No. 12813-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF/ICC, Indirect ELISA

Synonyms

LTrpC 6, LTrpC6, LTrpC-6, Trp p8, TRPP8

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

12813-1-PBS targets TRPM8 in IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag3483

Product name: Recombinant human TRPM8 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 3-192 aa of BC001135

Sequence: SFLPVHTIVLIRENVCKCGYAQSQHMEGTQINQSEKWNYKKHTKEFPTDAFGDIQFETLGKKGKYIRLSCDTDAEILYELLTQHWHLKTPNLVISVTGGAKNFALKPRMRKIFSRLIYIAQSKGAWILTGGTHYGLMKYIGEVVRDNTISRSSEENIVAIGIAAWGMVSNRDTLIRNCDAEVPVGQEEVC

相同性解析による交差性が予測される生物種
Full Name transient receptor potential cation channel, subfamily M, member 8
Calculated molecular weight 1104 aa, 128 kDa
GenBank accession numberBC001135
Gene Symbol TRPM8
Gene ID (NCBI) 79054
RRIDAB_2877883
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ7Z2W7
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

TRPM8, also known as CMR1 (cold-menthol receptor-1), is a nonselective cation channel activated by cold and the cooling substances menthol and icilin (PMID: 11893340; 11882888). It serves as a neuronal sensor of cold temperatures. Functioning TRPM8 channels are required for behavioral responses to innocuous cool, noxious cold, injury-evoked cold hypersensitivity, and cooling-mediated analgesia (PMID: 17538622; 21438154; 16920620; 21984952). Additionally, TRPM8 has also been reported to be involved in thermoregulation and the regulation of basal tear flow (PMID: 21407809; 21076394). TRPM8 is expressed extensively in sensory nerves, human prostate and overexpressed in a variety of cancers including prostate, breast, lung, colon and skin melanomas (PMID: 11325849; 20816009).

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