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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TRPC6 Recombinant monoclonal antibody (84517-5-RR)

    WB analysis using 84517-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84517-5-RR (TRPC6 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using TRPC6 Recombinant monoclonal antibody (84517-5-RR)

    IF Staining of A549 using 84517-5-RR

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using TRPC6 antibody (84517-5-RR, Clone: 251552A8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

TRPC6 Recombinant monoclonal antibody

TRPC6 Uni-rAb® Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 84517-5-RR
Clone No.251552A8

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Synonyms

FSGS2, Transient receptor protein 6, TRP 6, TRP6, TRP-6

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA549 cells, COLO 320 cells, mouse lung tissue, human placenta tissue
Positive IF/ICC detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84517-5-RR targets TRPC6 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

Peptide

相同性解析による交差性が予測される生物種
Full Name transient receptor potential cation channel, subfamily C, member 6
Calculated molecular weight 931 aa, 106 kDa
Observed molecular weight130 kDa
GenBank accession numberBC093658
Gene Symbol TRPC6
Gene ID (NCBI) 7225
RRIDAB_3743577
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ9Y210
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Transient receptor potential canonical (TRPC) channels mediate the influx of different types of cations through the cell membrane and are involved in many functions of the organism (PMID: 28508319). The TRPC subfamily consists of seven members, TRPC1, TRPC2, TRPC3, TRPC4, TRPC5, TRPC6, TRPC7 (PMID: 32872338). TRPC6 is a Ca(2+)-permeable non-selective cation channel expressed in most human tissues. TRPC6 has been considered as potential therapeutic targets for malignant glioma treatment (PMID: 35489424).

Protocols

Product Specific Protocols
IF protocol for TRPC6 antibody 84517-5-RRDownload protocol
WB protocol for TRPC6 antibody 84517-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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