Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse brain tissue, rat brain tissue, mouse cerebellum tissue, rat cerebellum tissue, pig brain tissue, fetal human brain tissue |
| Positive IP detected in | rat brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
87978-1-RR targets TRPC5 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat, pig samples.
| Tested Reactivity | human, mouse, rat, pig |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag23271 Product name: Recombinant human TRPC5 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 756-973 aa of BC137271 Sequence: EVLDLLGNRKHPRSFSTSSTELSQRDDNNDGSGGARAKSKSVSFNLGCKKKTCHGPPLIRTMPRSSGAQGKSKAESSSKRSFMGPSLKKLGLLFSKFNGHMSEPSSEPMYTISDGIVQQHCMWQDIRYSQMEKGKAEACSQSEINLSEVELGEVQGAAQSSECPLACSSSLHCASSICSSNSKLLDSSEDVFETWGEACDLLMHKWGDGQEEQVTTRL 相同性解析による交差性が予測される生物種 |
| Full Name | transient receptor potential cation channel, subfamily C, member 5 |
| Calculated molecular weight | 111 kDa |
| Observed molecular weight | 100-111 kDa |
| GenBank accession number | BC137271 |
| Gene Symbol | TRPC5 |
| Gene ID (NCBI) | 7224 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q9UL62 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Short transient receptor potential channel 5 (TRPC5) is abundantly expressed in hippocampal neurons (PMID: 9553080). TRPC5 channels participate in a variety of physiological and pathophysiological processes, covering vascular smooth muscle activity, endothelial function, adiponectin modulation, oxidative stress, epilepsy, anxiety, pain sensation, as well as cardiac remodeling (PMID: 15538613; 22668831; 16685599; 26069213).
Protocols
| Product Specific Protocols | |
|---|---|
| IP protocol for TRPC5 antibody 87978-1-RR | Download protocol |
| WB protocol for TRPC5 antibody 87978-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |