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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TRPA1 Polyclonal antibody (25226-1-AP)

    WB analysis using 25226-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25226-1-AP (TRPA1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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TRPA1 Polyclonal antibody

TRPA1 Polyclonal Antibody for WB, ELISA
Cat No. 25226-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

Wasabi receptor, p120, ANKTM1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive WB detected inA549 cells, U2OS cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25226-1-AP targets TRPA1 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TRPA1 fusion protein Ag18685 相同性解析による交差性が予測される生物種
Full Name transient receptor potential cation channel, subfamily A, member 1
Calculated molecular weight 1119 aa, 128 kDa
Observed molecular weight130 kDa
GenBank accession numberBC153003
Gene Symbol TRPA1
Gene ID (NCBI) 8989
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO75762
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TRPA1 (Transient Receptor Potential Ankyrin 1) is a member of the transient receptor potential (TRP) channel family and is a non-selective cation channel. TRPA1 is predominantly expressed in sensory neurons and is involved in a variety of physiological activities, such as stabilizing cellular membrane potentials, maintaining cellular fluid homeostasis, and regulating intercellular signaling. TRPA1 is a multimodal cell membrane receptor that senses different stimuli and upon activation generates action potential signals via osmolality, temperature and inflammatory factors. TRPA1 has been associated with neurogenic inflammation, osteoarthritis, atopic dermatitis, asthma, inflammatory bowel disease, migraine headache, cancer pain, gout and other diseases, and it is a promising target for drug therapy.

Protocols

Product Specific Protocols
WB protocol for TRPA1 antibody 25226-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols