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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using TROVE2 Monoclonal antibody (67149-1-Ig)

    WB analysis of HeLa using 67149-1-Ig (same clone as 67149-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 67149-1-Ig (TROVE2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67149-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using TROVE2 Monoclonal antibody (67149-1-Ig)

    WB analysis of HEK-293 using 67149-1-Ig (same clone as 67149-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 67149-1-Ig (TROVE2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67149-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using TROVE2 Monoclonal antibody (67149-1-Ig)

    WB analysis of MCF-7 using 67149-1-Ig (same clone as 67149-1-PBS)

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 67149-1-Ig (TROVE2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67149-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using TROVE2 Monoclonal antibody (67149-1-Ig)

    WB analysis of Jurkat using 67149-1-Ig (same clone as 67149-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 67149-1-Ig (TROVE2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67149-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using TROVE2 Monoclonal antibody (67149-1-Ig)

    IHC staining of mouse brain using 67149-1-Ig (same clone as 67149-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67149-1-Ig (TROVE2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67149-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using TROVE2 Monoclonal antibody (67149-1-Ig)

    IHC staining of mouse brain using 67149-1-Ig (same clone as 67149-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67149-1-Ig (TROVE2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67149-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using TROVE2 Monoclonal antibody (67149-1-Ig)

    IHC staining of mouse brain using 67149-1-Ig (same clone as 67149-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67149-1-Ig (TROVE2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67149-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using TROVE2 Monoclonal antibody (67149-1-Ig)

    IHC staining of mouse brain using 67149-1-Ig (same clone as 67149-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67149-1-Ig (TROVE2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67149-1-PBS in a different storage buffer formulation.

TROVE2 Monoclonal antibody, PBS Only

TROVE2 Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 67149-1-PBS
Clone No.2A4E5

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, Indirect ELISA

Synonyms

60 kDa ribonucleoprotein Ro, 60 kDa Ro protein, Ro 60 kDa autoantigen, RO60, RoRNP, Sjoegren syndrome antigen A2, SS A, SSA2, TROVE domain family member 2, TROVE domain family, member 2, TROVE2

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67149-1-PBS targets TROVE2 in WB, IHC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag4379

Product name: Recombinant human TROVE2 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 21-369 aa of BC036658

Sequence: DGYVWQVTDMNRLHRFLCFGSEGGTYYIKEQKLGLENAEALIRLIEDGRGCEVIQEIKSFSQEGRTTKQEPMLFALAICSQCSDISTKQAAFKAVSEVCRIPTHLFTFIQFKKDLKESMKCGMWGRALRKAIADWYNEKGGMALALAVTKYKQRNGWSHKDLLRLSHLKPSSEGLAIVTKYITKGWKEVHELYKEKALSVETEKLLKYLEAVEKVKRTRDELEVIHLIEEHRLVREHLLTNHLKSKEVWKALLQEMPLTALLRNLGKMTANSVLEPGNSEVSLVCEKLCNEKLLKKARIHPFHILIALETYKTGHGLRGKLKWRPDEEILKALDAAFYKTFKTVEPTGK

相同性解析による交差性が予測される生物種
Full Name TROVE domain family, member 2
Calculated molecular weight 60 kDa
Observed molecular weight 60 kDa
GenBank accession numberBC036658
Gene Symbol TROVE2
Gene ID (NCBI) 6738
RRIDAB_2882447
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP10155
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

TROVE2, also named as RO60 or RoRNP, is a 538 amino acid protein, which is localized in cytoplasmic mRNP granules containing untranslated mRNAs. TROVE2 as a RNA-binding protein that binds to misfolded non-coding RNAs, pre-5S rRNA, and several small cytoplasmic RNA molecules known as Y RNAs. TROVE2 may stabilize some of these RNAs and protect them from degradation (PubMed:18056422). TROVE2 binds to endogenous Alu retroelements which are induced by type I IFN and stimulate porinflammaotry cytokine secretion.

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