Validation Data Gallery
Tested Applications
| Positive WB detected in | A549 cells, HeLa cells, NIH/3T3 cells, mouse kidney tissue, rat kidney tissue |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
31906-1-AP targets TRNT1 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag36149 Product name: Recombinant human TRNT1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 271-405 aa of BC012537 Sequence: FKVQDDVTKLDLRLKIAKEEKNLGLFIVKNRKDLIKATDSSDPLKPYQDFIIDSREPDATTRVCELLKYQGEHCLLKEMQQWSIPPFPVSGHDIRKVGISSGKEIGALLQQLREQWKKSGYQMEKDELLSYIKKT 相同性解析による交差性が予測される生物種 |
| Full Name | tRNA nucleotidyl transferase, CCA-adding, 1 |
| Calculated molecular weight | 50 kDa |
| Observed molecular weight | 45 kDa |
| GenBank accession number | BC012537 |
| Gene Symbol | TRNT1 |
| Gene ID (NCBI) | 51095 |
| RRID | AB_3670139 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | Q96Q11 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
tRNA nucleotidyl transferase 1 (TRNT1, also known as CCA1, RPEM, SIFD, MtCCA, and CGI-47 ) is a cytosine-cytosine-adenine (CCA) - adding enzyme which belongs to the tRNA nucleotidyltransferase/poly (A) polymerase family. It can catalyze the addition of the conserved nucleotide triplet CCA to the 3' terminus of tRNA molecules (PMID: 27370603). Mutations in TRNT1 would cause congenital sideroblastic anemia with immunodeficiency, fevers, and developmental delay (SIFD) (PMID: 25193871).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for TRNT1 antibody 31906-1-AP | Download protocol |
| WB protocol for TRNT1 antibody 31906-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |