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Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of mouse brain tissue using TRNP1 Recombinant antibody (83410-4-RR)

    IHC staining of mouse brain using 83410-4-RR (same clone as 83410-4-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83410-4-RR (TRNP1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83410-4-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using TRNP1 Recombinant antibody (83410-4-RR)

    IHC staining of mouse brain using 83410-4-RR (same clone as 83410-4-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83410-4-RR (TRNP1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83410-4-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using TRNP1 Recombinant antibody (83410-4-RR)

    IF Staining of mouse brain using 83410-4-RR (same clone as 83410-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using TRNP1 antibody (83410-4-RR, Clone: 240331B6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83410-4-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using TRNP1 Recombinant antibody (83410-4-RR)

    IF Staining of HeLa using 83410-4-RR (same clone as 83410-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TRNP1 antibody (83410-4-RR, Clone: 240331B6 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83410-4-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using TRNP1 Recombinant antibody (83410-4-RR)

    FC experiment of HeLa using 83410-4-RR (same clone as 83410-4-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug TRNP1 Recombinant antibody (83410-4-RR, Clone:240331B6) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83410-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83410-4-RR

    Affinity and Kinetic Characterization of 83410-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83410-4-RR against Human TRNP1 were performed. The affinity constant is 2.5 nM.

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TRNP1 Recombinant antibody, PBS Only

TRNP1 Uni-rAbTM Recombinant Antibody for IHC, IF/ICC, IF-P, ELISA
Cat No. 83410-4-PBS
Clone No.240331B6

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF/ICC, IF-P, ELISA

Synonyms

TRNP, TNRP, TMF-regulated nuclear protein 1, C1orf225, 240331B6

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83410-4-PBS targets TRNP1 in IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen TRNP1 fusion protein Ag22504 相同性解析による交差性が予測される生物種
Full Name TMF1-regulated nuclear protein 1
Calculated molecular weight 227 aa, 23 kDa
GenBank accession numberBC069216
Gene Symbol TRNP1
Gene ID (NCBI) 388610
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ6NT89
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

TRNP1 (TMF-regulated nuclear protein 1), also called C1orf225, is a DNA-binding factor that regulates the expression of a subset of genes and plays a key role in tangential, radial, and lateral expansion of the brain neocortex (PMID: 27264089; 23622239; 36947129). It has been shown to exert potent roles in neural development affecting neural stem cell self-renewal and brain folding (PMID: 32627867). It may also control the proliferation rate of cells by regulating their progression through key cell-cycle transition points.