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Species Reactivity
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TRNT1 Polyclonal antibody

TRNT1 Polyclonal Antibody for IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 25376-1-AP

Synonyms

MTO2, MTO2 homolog, MTU1, TRMT, TRMT1, TRMU, TRNT1

Validation Data Gallery

Applications:
  • IHC staining of mouse skeletal muscle using 25376-1-AP

    IHC staining of mouse skeletal muscle using 25376-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 25376-1-AP (TRMU antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse skeletal muscle using 25376-1-AP

    IHC staining of mouse skeletal muscle using 25376-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 25376-1-AP (TRMU antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HepG2 using 25376-1-AP

    IF Staining of HepG2 using 25376-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 25376-1-AP (TRMU antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive IHC detected inmouse skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

25376-1-AP targets TRNT1 in IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TRNT1 fusion protein Ag21930 相同性解析による交差性が予測される生物種
Full Name tRNA 5-methylaminomethyl-2-thiouridylate methyltransferase
Calculated molecular weight 421 aa, 48 kDa
GenBank accession numberBC027991
Gene symbol TRMU
Gene ID (NCBI) 55687
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IHC protocol for TRNT1 antibody 25376-1-APDownload protocol
IF protocol for TRNT1 antibody 25376-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols