Validation Data Gallery
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Tested Applications
| Positive WB detected in | 5637 cells, RAW 264.7 cells, MCF-7 cells, U-251 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
31169-1-AP targets TRMT61A in WB, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | TRMT61A fusion protein Ag34396 相同性解析による交差性が予測される生物種 |
| Full Name | tRNA methyltransferase 61 homolog A (S. cerevisiae) |
| Observed molecular weight | 30-35 kDa |
| GenBank accession number | BC010167 |
| Gene Symbol | TRMT61A |
| Gene ID (NCBI) | 115708 |
| RRID | AB_3669880 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | Q96FX7 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
N1-methyladenosine (m1A) is an important posttranscriptional modification and occurs throughout the tRNA structure.m1A at position 58 (m1A58) is the predominant m1A modification of cytoplasmic tRNA in eukaryotic cells. The formation of m1A58 on cytoplasmic tRNAs is mediated by the methyltransferase complex TRMT6/TRMT61A, in which TRMT61A functions as the catalytic subunit and TRMT6 is responsible for tRNA binding.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for TRMT61A antibody 31169-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |