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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TRMT6 Recombinant antibody (83843-6-RR)

    WB analysis using 83843-6-RR (same clone as 83843-6-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83843-6-RR (TRMT6 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83843-6-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using TRMT6 Recombinant antibody (83843-6-RR)

    WB analysis of HepG2 using 83843-6-RR (same clone as 83843-6-PBS)

    WB result of TRMT6 antibody (83843-6-RR; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TRMT6 transfected HepG2 cells. This data was developed using the same antibody clone with 83843-6-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovarian cancer using TRMT6 Recombinant antibody (83843-6-RR)

    IHC staining of human ovarian cancer using 83843-6-RR (same clone as 83843-6-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 83843-6-RR (TRMT6 antibody) at dilution of 1:1200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83843-6-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using TRMT6 Recombinant antibody (83843-6-RR)

    IF Staining of A431 using 83843-6-RR (same clone as 83843-6-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using TRMT6 antibody (83843-6-RR, Clone: 240911D3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83843-6-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83843-6-RR

    Affinity and Kinetic Characterization of 83843-6-RR

    Biolayer interferometry (BLl) kinetic assays of 83843-6-RR against Human TRMT6 were performed. The affinity constant is below 1 pM.

  • Featured Product
  • KD/KO Validated

TRMT6 Recombinant monoclonal antibody, PBS Only

TRMT6 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 83843-6-PBS
Clone No.240911D3

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

240911D3, CGI 09, CGI-09, mRNA methyladenosine-N(1)-methyltransferase non-catalytic subunit TRM6, TRM6

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83843-6-PBS targets TRMT6 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag10176

Product name: Recombinant human TRMT6 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-497 aa of BC001262

Sequence: MEGSGEQPGPQPQHPGDHRIRDGDFVVLKREDVFKAVQVQRRKKVTFEKQWFYLDNVIGHSYGTAFEVTSGGSLQPKKKREEPTAETKEAGTDNRNIVDDGKSQKLTQDDIKALKDKGIKGEEIVQQLIENSTTFRDKTEFAQDKYIKKKKKKYEAIITVVKPSTRILSIMYYAREPGKINHMRYDTLAQMLTLGNIRAGNKMIVMETCAGLVLGAMMERMGGFGSIIQLYPGGGPVRAATACFGFPKSFLSGLYEFPLNKVDSLLHGTFSAKMLSSEPKDSALVEESNGTLEEKQASEQENEDSMAEAPESNHPEDQETMETISQDPEHKGPKERGSKKDYIQEKQRRQEEQRKRHLEAAALLSERNADGLIVASRFHPTPLLLSLLDFVAPSRPFVVYCQYKEPLLECYTKLRERGGVINLRLSETWLRNYQVLPDRSHPKLLMSGGGGYLLSGFTVAMDNLKADTSLKSNASTLESHETEEPAAKKRKCPESDS

相同性解析による交差性が予測される生物種
Full Name tRNA methyltransferase 6 homolog (S. cerevisiae)
Calculated molecular weight 56 kDa
Observed molecular weight 55-60 kDa
GenBank accession numberBC001262
Gene Symbol TRMT6
Gene ID (NCBI) 51605
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purfication
UNIPROT IDQ9UJA5
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Transfer RNA (tRNA) modifications help regulate the efficiency of mRNA translation by maintaining the correct reading frames within mRNA. TRMT6 (tRNA methyltransferase 6 ), also known as GCD10 or TRM6, is a 497 amino acid protein that localizes to the nucleus and exists as a heterodimer with TRM61. Expressed in liver, brain, ovary and testis, TRMT6 functions as a substrate-binding subunit of tRNA and is thought to catalyze the formation of N(1)-methyladenine at position 58 in initiator methionyl-tRNA. TRMT6 exists as three alternatively spliced isoforms that, in response to DNA damage, may be phosphorylated by ATM or ATR.