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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TRMT6 Recombinant antibody (83843-5-RR)

    WB analysis using 83843-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83843-5-RR (TRMT6 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using TRMT6 Recombinant antibody (83843-5-RR)

    WB analysis of HepG2 using 83843-5-RR

    WB result of TRMT6 antibody (83843-5-RR; 1:60000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TRMT6 transfected HepG2 cells.

  • Immunohistochemistry (IHC) staining of human ovarian cancer using TRMT6 Recombinant antibody (83843-5-RR)

    IHC staining of human ovarian cancer using 83843-5-RR

    Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 83843-5-RR (TRMT6 antibody) at dilution of 1:3000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using TRMT6 Recombinant antibody (83843-5-RR)

    IF Staining of HeLa using 83843-5-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TRMT6 antibody (83843-5-RR, Clone: 240911C5 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using TRMT6 Recombinant antibody (83843-5-RR)

    IF Staining of A431 using 83843-5-RR

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using TRMT6 antibody (83843-5-RR, Clone: 240911C5 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 83843-5-RR

    Affinity and Kinetic Characterization of 83843-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83843-5-RR against Human TRMT6 were performed. The affinity constant is below 1 pM.

  • Featured Product
  • KD/KO Validated

TRMT6 Recombinant monoclonal antibody

TRMT6 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 83843-5-RR
Clone No.240911C5

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

TRMT 6, TRM6, mRNA methyladenosine-N(1)-methyltransferase non-catalytic subunit TRM6, CGI-09, CGI 09

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

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Tested Applications

Positive WB detected inU-251 cells, HepG2 cells, U-87 MG cells, SMMC-7721 cells, HuH-7 cells
Positive IHC detected inhuman ovarian cancer
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells, A431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1500-1:6000
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83843-5-RR targets TRMT6 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag10176

Product name: Recombinant human TRMT6 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-497 aa of BC001262

Sequence: MEGSGEQPGPQPQHPGDHRIRDGDFVVLKREDVFKAVQVQRRKKVTFEKQWFYLDNVIGHSYGTAFEVTSGGSLQPKKKREEPTAETKEAGTDNRNIVDDGKSQKLTQDDIKALKDKGIKGEEIVQQLIENSTTFRDKTEFAQDKYIKKKKKKYEAIITVVKPSTRILSIMYYAREPGKINHMRYDTLAQMLTLGNIRAGNKMIVMETCAGLVLGAMMERMGGFGSIIQLYPGGGPVRAATACFGFPKSFLSGLYEFPLNKVDSLLHGTFSAKMLSSEPKDSALVEESNGTLEEKQASEQENEDSMAEAPESNHPEDQETMETISQDPEHKGPKERGSKKDYIQEKQRRQEEQRKRHLEAAALLSERNADGLIVASRFHPTPLLLSLLDFVAPSRPFVVYCQYKEPLLECYTKLRERGGVINLRLSETWLRNYQVLPDRSHPKLLMSGGGGYLLSGFTVAMDNLKADTSLKSNASTLESHETEEPAAKKRKCPESDS

相同性解析による交差性が予測される生物種
Full Name tRNA methyltransferase 6 homolog (S. cerevisiae)
Calculated molecular weight 56 kDa
Observed molecular weight 55-60 kDa
GenBank accession numberBC001262
Gene Symbol TRMT6
Gene ID (NCBI) 51605
RRIDAB_3671427
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purfication
UNIPROT IDQ9UJA5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Transfer RNA (tRNA) modifications help regulate the efficiency of mRNA translation by maintaining the correct reading frames within mRNA. TRMT6 (tRNA methyltransferase 6 ), also known as GCD10 or TRM6, is a 497 amino acid protein that localizes to the nucleus and exists as a heterodimer with TRM61. Expressed in liver, brain, ovary and testis, TRMT6 functions as a substrate-binding subunit of tRNA and is thought to catalyze the formation of N(1)-methyladenine at position 58 in initiator methionyl-tRNA.

Protocols

Product Specific Protocols
WB protocol for TRMT6 antibody 83843-5-RRDownload protocol
IHC protocol for TRMT6 antibody 83843-5-RRDownload protocol
IF protocol for TRMT6 antibody 83843-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols