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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TRMT6 Polyclonal antibody (16727-1-AP)

    WB analysis using 16727-1-AP (same clone as 16727-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 16727-1-AP (TRMT6 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16727-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using TRMT6 Polyclonal antibody (16727-1-AP)

    WB analysis of HepG2 using 16727-1-AP (same clone as 16727-1-PBS)

    WB result of TRMT6 antibody (16727-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TRMT6 transfected HepG2 cells. This data was developed using the same antibody clone with 16727-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using TRMT6 Polyclonal antibody (16727-1-AP)

    IP experiment of HeLa using 16727-1-AP (same clone as 16727-1-PBS)

    IP result of anti-TRMT6 (IP:16727-1-AP, 4ug; Detection:16727-1-AP 1:700) with HeLa cells lysate 2000 ug. This data was developed using the same antibody clone with 16727-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using TRMT6 Polyclonal antibody (16727-1-AP)

    IHC staining of human colon cancer using 16727-1-AP (same clone as 16727-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16727-1-AP (TRMT6 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16727-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using TRMT6 Polyclonal antibody (16727-1-AP)

    IHC staining of human colon cancer using 16727-1-AP (same clone as 16727-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16727-1-AP (TRMT6 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16727-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

TRMT6 Polyclonal antibody, PBS Only

TRMT6 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 16727-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

tRNA (adenine(58)-N(1))-methyltransferase non-catalytic subunit TRM6, TRMT 6, TRM6, mRNA methyladenosine-N(1)-methyltransferase non-catalytic subunit TRM6, CGI-09

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

This antibody works well in ProteinSimple system.

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

16727-1-PBS targets TRMT6 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag10176

Product name: Recombinant human TRMT6 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-497 aa of BC001262

Sequence: MEGSGEQPGPQPQHPGDHRIRDGDFVVLKREDVFKAVQVQRRKKVTFEKQWFYLDNVIGHSYGTAFEVTSGGSLQPKKKREEPTAETKEAGTDNRNIVDDGKSQKLTQDDIKALKDKGIKGEEIVQQLIENSTTFRDKTEFAQDKYIKKKKKKYEAIITVVKPSTRILSIMYYAREPGKINHMRYDTLAQMLTLGNIRAGNKMIVMETCAGLVLGAMMERMGGFGSIIQLYPGGGPVRAATACFGFPKSFLSGLYEFPLNKVDSLLHGTFSAKMLSSEPKDSALVEESNGTLEEKQASEQENEDSMAEAPESNHPEDQETMETISQDPEHKGPKERGSKKDYIQEKQRRQEEQRKRHLEAAALLSERNADGLIVASRFHPTPLLLSLLDFVAPSRPFVVYCQYKEPLLECYTKLRERGGVINLRLSETWLRNYQVLPDRSHPKLLMSGGGGYLLSGFTVAMDNLKADTSLKSNASTLESHETEEPAAKKRKCPESDS

相同性解析による交差性が予測される生物種
Full Name tRNA methyltransferase 6 homolog (S. cerevisiae)
Calculated molecular weight 56 kDa
Observed molecular weight 55-60 kDa
GenBank accession numberBC001262
Gene Symbol TRMT6
Gene ID (NCBI) 51605
RRIDAB_2878306
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UJA5
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Transfer RNA (tRNA) modifications help regulate the efficiency of mRNA translation by maintaining the correct reading frames within mRNA. TRMT6 (tRNA methyltransferase 6 ), also known as GCD10 or TRM6, is a 497 amino acid protein that localizes to the nucleus and exists as a heterodimer with TRM61. Expressed in liver, brain, ovary and testis, TRMT6 functions as a substrate-binding subunit of tRNA and is thought to catalyze the formation of N(1)-methyladenine at position 58 in initiator methionyl-tRNA. TRMT6 exists as three alternatively spliced isoforms that, in response to DNA damage, may be phosphorylated by ATM or ATR. This antibody is specific to 56kd TRMT6 protein.

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