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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TRMT12 Recombinant antibody (84167-3-RR)

    WB analysis using 84167-3-RR (same clone as 84167-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84167-3-RR (TRMT12 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84167-3-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using TRMT12 Recombinant antibody (84167-3-RR)

    WB analysis of MCF-7 using 84167-3-RR (same clone as 84167-3-PBS)

    WB result of TRMT12 antibody (84167-3-RR; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-TRMT12 transfected MCF-7 cells. This data was developed using the same antibody clone with 84167-3-PBS in a different storage buffer formulation.

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  • KD/KO Validated

TRMT12 Recombinant monoclonal antibody, PBS Only

TRMT12 Uni-rAb® Recombinant Antibody for WB, Indirect ELISA
Cat No. 84167-3-PBS
Clone No.241483H1

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Indirect ELISA

Synonyms

241483H1, EC:2.5.1.114, TRM12, tRNA methyltransferase 12, tRNA wybutosine-synthesizing protein 2 homolog

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84167-3-PBS targets TRMT12 in WB, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag9258

Product name: Recombinant human TRMT12 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 99-448 aa of BC011713

Sequence: QGCSPAQKLCLEVSRWVEGRGVKWSAELEADLPRSWQRHGNLLLLSEDCFQAKQWKNLGPELWETVALALGVQRLAKRGRVSPDGTRTPAVTLLLGDHGWVEHVDNGIRYKFDVTQCMFSFGNITEKLRVASLSCAGEVLVDLYAGIGYFTLPFLVHAGAAFVHACEWNPHAVVALRNNLEINGVADRCQIHFGDNRKLKLSNIADRVILGLIPSSEEGWPIACQVLRQDAGGILHIHQNVESFPGKNLQALGVSKVEKEHWLYPQQITTNQWKNGATRDSRGKMLSPATKPEWQRWAESAETRIATLLQQVHGKPWKTQILHIQPVKSYAPHVDHIVLDLECCPCPSVG

相同性解析による交差性が予測される生物種
Full Name tRNA methyltransferase 12 homolog (S. cerevisiae)
Calculated molecular weight 448 aa, 50 kDa
Observed molecular weight 45-50 kDa
GenBank accession numberBC011713
Gene Symbol TRMT12
Gene ID (NCBI) 55039
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purfication
UNIPROT IDQ53H54
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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