Validation Data Gallery
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Tested Applications
| Positive WB detected in | HEK-293 cells, HeLa cells, K-562 cells, PC-3 cells, SH-SY5Y cells |
| Positive IHC detected in | human ovary tumor tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
26472-1-AP targets TRMT112 in WB, IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag24126 Product name: Recombinant human HSPC152 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 40-125 aa of BC017172 Sequence: NFVARMIPKVEWSAFLEAADNLRLIQVPKGPVEGYEENEEFLRTMHHLLLEVEVIEGTLQCPESGRMFPISRGIPNMLLSEEETES 相同性解析による交差性が予測される生物種 |
| Full Name | hypothetical protein HSPC152 |
| Observed molecular weight | 14 kDa |
| GenBank accession number | BC017172 |
| Gene Symbol | TRMT112 |
| Gene ID (NCBI) | 51504 |
| RRID | AB_3085875 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UI30 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
TRMT112 is a small evolutionarily conserved protein that acts as a co-factor and activator for different MTases involved in rRNA, tRNA and protein methylation. The TRMT112 interaction with 18S rRNA MTases WBSCR22 (BUD23, MERM1) and METTL5 is required for their metabolic stability and enzymatic activity in cells. (PMID: 34948388, PMID: 34948388)
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for TRMT112 antibody 26472-1-AP | Download protocol |
| WB protocol for TRMT112 antibody 26472-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |