Validation Data Gallery
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Tested Applications
| Positive WB detected in | LNCaP cells, HeLa cells, HepG2 cells, K-562 cells, PC-12 cells, rat heart tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
84991-4-RR targets TRMT10C in WB, ELISA applications and shows reactivity with human, rat samples.
| Tested Reactivity | human, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | TRMT10C fusion protein Ag30642 相同性解析による交差性が予測される生物種 |
| Full Name | RNA (guanine-9-) methyltransferase domain containing 1 |
| Calculated molecular weight | 47 kDa |
| Observed molecular weight | 45 kDa |
| GenBank accession number | NM_017819 |
| Gene Symbol | TRMT10C |
| Gene ID (NCBI) | 54931 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q7L0Y3 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
tRNA methyltransferase 10 homolog C (TRMT10C, also known as MRPP1) is involved in mitochondrial RNA processing and modification (PMID: 21593607). It is essential for the 5'-ends processing of mitochondrial tRNAs and plays a critical role in mitochondrial respiration and translation (PMID: 18984158; 29040705). Mutations in TRMT10C may be a cause of mitochondrial disease and highlight the importance of RNA processing for correct mitochondrial function (PMID: 27132592).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for TRMT10C antibody 84991-4-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |