TRMT10C Polyclonal antibody
TRMT10C Polyclonal Antibody for WB, IF, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human, Mouse, Rat and More (2)
Applications
WB, IF, IHC, ELISA
Conjugate
Unconjugated
Cat no : 29087-1-AP
Synonyms
Validation Data Gallery
Applications:
Tested Applications
| Positive WB detected in | HepG2 cells, PC-12 cells, mouse heart tissue, rat heart tissue |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | U2OS cells |
Planning an IHC experiment? We recommend our IHCeasy TRMT10C Ready-To-Use IHC Kit. TRMT10C primary antibody included.
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:2000-1:8000 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| WB | See 2 publications below |
Product Information
29087-1-AP targets TRMT10C in WB, IF, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.
| Tested Reactivity | Human, Mouse, Rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | TRMT10C fusion protein Ag30642 相同性解析による交差性が予測される生物種 |
| Full Name | RNA (guanine-9-) methyltransferase domain containing 1 |
| Calculated molecular weight | 47 kDa |
| Observed molecular weight | 40-45 kDa |
| GenBank accession number | NM_017819 |
| Gene symbol | RG9MTD1 |
| Gene ID (NCBI) | 54931 |
| RRID | AB_2881239 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for TRMT10C antibody 29087-1-AP | Download protocol |
| IHC protocol for TRMT10C antibody 29087-1-AP | Download protocol |
| IF protocol for TRMT10C antibody 29087-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Death Discov N1-Methyladenosine modification of mRNA regulates neuronal gene expression and oxygen glucose deprivation/reoxygenation induction | ||
Circulation Stimulates Short-Chain Dehydrogenase/Reductase Proteins to Alleviate Heart Failure Independent of Mitochondrial Protein Deacetylation |
