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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TRMT1 Recombinant antibody (85897-5-RR)

    WB analysis using 85897-5-RR (same clone as 85897-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85897-5-RR (TRMT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85897-5-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using TRMT1 Recombinant antibody (85897-5-RR)

    IF Staining of HeLa using 85897-5-RR (same clone as 85897-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TRMT1 antibody (85897-5-RR, Clone: 250178D5 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85897-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 85897-5-RR

    Affinity and Kinetic Characterization of 85897-5-RR

    Biolayer interferometry (BLl) kinetic assays of 85897-5-RR against Human TRMT1 were performed. The affinity constant is 26.2 pM.

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TRMT1 Recombinant antibody, PBS Only

TRMT1 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 85897-5-PBS
Clone No.250178D5

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

EC:2.1.1.216, TRM1, TRMT 1, tRNA (guanine(26)-N(2))-dimethyltransferase, tRNA 2,2-dimethylguanosine-26 methyltransferase

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

85897-5-PBS targets TRMT1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen TRMT1 fusion protein Ag6837 相同性解析による交差性が予測される生物種
Full Name TRM1 tRNA methyltransferase 1 homolog (S. cerevisiae)
Calculated molecular weight 81 kDa
Observed molecular weight75 kDa
GenBank accession numberBC002492
Gene Symbol TRMT1
Gene ID (NCBI) 55621
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9NXH9
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

TRMT1 is an RNA methyltransferase responsible for the formation of N2,N2-dimethylguanosine (m2,2G) at position 26 in both cytosolic and mitochondrial tRNAs.TRMT1-catalyzed tRNA modifications are important for maintenance of global protein translation levels including proteins involved in cellular growth, development, and stress response. Human neural stem cells with TRMT1 knockdowns were found to have hypersensitivity to redox stress, implicating TRMT1 and the m2,2G26 modification in the regulation of redox homeostasis.