Validation Data Gallery
Tested Applications
| Positive WB detected in | HT-1080 cells, mouse testis tissue, Jurkat cells |
| Positive IP detected in | mouse testis tissue |
It works great for western. By Dr. Soory
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 9 publications below |
| WB | See 20 publications below |
| IHC | See 6 publications below |
| IF | See 8 publications below |
| IP | See 1 publications below |
| CoIP | See 1 publications below |
Product Information
19602-1-AP targets TRIP13 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag6595 Product name: Recombinant human TRIP13 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-301 aa of BC000404 Sequence: MDEAVGDLKQALPCVAESPTVHVEVHQRGSSTAKKEDINLSVRKLLNRHNIVFGDYTWTEFDEPFLTRNVQSVSIIDTELKVKDSQPIDLSACTVALHIFQLNEDGPSSENLEEETENIIAANHWVLPAAEFHGLWDSLVYDVEVKSHLLDYVMTTLLFSDKNVNSNLITWNRVVLLHGPPGTGKTSLCKALAQKLTIRLSSRYRYGQLIEINSHSLFSKWFSESGKLVTKMFQKIQDLIDDKDALVFVLIDEVESLTAARNACRAGTEPSDAIRVVNAVLTQIDQIKRHSNVVILTTSNI 相同性解析による交差性が予測される生物種 |
| Full Name | thyroid hormone receptor interactor 13 |
| Calculated molecular weight | 49 kDa |
| Observed molecular weight | 49 kDa |
| GenBank accession number | BC000404 |
| Gene Symbol | TRIP13 |
| Gene ID (NCBI) | 9319 |
| RRID | AB_10642702 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q15645 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
TRIP13 gene encodes thyroid receptor-interacting protein 13, that interact with thyroid hormone receptors, also known as human papillomavirus type 16 E1 protein-binding protein (16E1BP). This protein belongs to the AAA ATPase family and the PCH2 subfamily. TRIP13 gene may be one of the several playing roles in early-stage non-small cell lung cancer. TRIP13 also functions in chromosome recombination and chromosome structure development during meiosis, and it is required for development of higher-order chromosome structures and is needed for synaptonemal-complex formation.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for TRIP13 antibody 19602-1-AP | Download protocol |
| IP protocol for TRIP13 antibody 19602-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Adv Res Tetrahydrocurcumin targets TRIP13 inhibiting the interaction of TRIP13/USP7/c-FLIP to mediate c-FLIP ubiquitination in triple-negative breast cancer | ||
Nat Commun TRIP13 promotes error-prone nonhomologous end joining and induces chemoresistance in head and neck cancer.
| ||
Cell Prolif ZFP541 and KCTD19 regulate chromatin organization and transcription programs for male meiotic progression | ||
Mol Oncol TRIP13 promotes metastasis of colorectal cancer regardless of p53 and microsatellite instability status. | ||
Cell Death Dis TRIP13 promotes tumor growth and is associated with poor prognosis in colorectal cancer. |