Validation Data Gallery
Tested Applications
| Positive WB detected in | U2OS cells |
| Positive IHC detected in | mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
31976-1-AP targets TRIML2 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag37106 Product name: Recombinant human TRIML2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-155 aa of BC111959 Sequence: MVCGIQEAAENYRKLFQEILNTSREKLEAAKSILTDEQERMAMIQEEEQNFKKMIESEYSMRLRLLNEECEQNLQRQQECISDLNLRETLLNQAIKLATELEEMFQEMLQRLGRVGRENMEKLKESEARASEQVRSLLKLIVELEKKCGEGTLAL 相同性解析による交差性が予測される生物種 |
| Full Name | tripartite motif family-like 2 |
| Calculated molecular weight | 50 kDa |
| Observed molecular weight | 50 kDa |
| GenBank accession number | BC111959 |
| Gene Symbol | TRIML2 |
| Gene ID (NCBI) | 205860 |
| RRID | AB_3670164 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | Q8N7C3 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Tripartite motif family like 2 (TRIML2, also known as SPRYD6) is a member of the tripartite motif (TRIM) family of proteins. It probably interacts with p53 to facilitate the modification of p53 with small ubiquitin-like modifier 2 (SUMO2) (PMID: 25256710). By regulating p53, TRIML2 may also be an important factor in the pathogenesis of Alzheimer's disease (PMID: 27471163).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for TRIML2 antibody 31976-1-AP | Download protocol |
| WB protocol for TRIML2 antibody 31976-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |