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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TRIM59 Polyclonal antibody (28575-1-AP)

    WB analysis using 28575-1-AP (same clone as 28575-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 28575-1-AP (TRIM59 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28575-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using TRIM59 Polyclonal antibody (28575-1-AP)

    WB analysis using 28575-1-AP (same clone as 28575-1-PBS)

    rat spleen tissue were subjected to SDS PAGE followed by western blot with 28575-1-AP (TRIM59 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28575-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using TRIM59 Polyclonal antibody (28575-1-AP)

    IHC staining of human pancreas cancer using 28575-1-AP (same clone as 28575-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 28575-1-AP (TRIM59 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28575-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using TRIM59 Polyclonal antibody (28575-1-AP)

    IHC staining of human lung cancer using 28575-1-AP (same clone as 28575-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 28575-1-AP (TRIM59 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28575-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using TRIM59 Polyclonal antibody (28575-1-AP)

    IHC staining of human liver using 28575-1-AP (same clone as 28575-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 28575-1-AP (TRIM59 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28575-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using TRIM59 Polyclonal antibody (28575-1-AP)

    IHC staining of human breast cancer using 28575-1-AP (same clone as 28575-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 28575-1-AP (TRIM59 antibody) at dilution of 1:1000 (under 40x lens). This data was developed using the same antibody clone with 28575-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using TRIM59 Polyclonal antibody (28575-1-AP)

    IF Staining of U2OS using 28575-1-AP (same clone as 28575-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using TRIM59 antibody (28575-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 28575-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

TRIM59 Polyclonal antibody, PBS Only

TRIM59 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 28575-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

28575-1-PBS targets TRIM59 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag29560

Product name: Recombinant human TRIM59 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 128-326 aa of NM_173084

Sequence: GHPIDDLQSAYLKEKDTPQKLLEQLTDTHWTDLTHLIEKLKEQKSHSEKMIQGDKEAVLQYFKELNDTLEQKKKSFLTALCDVGNLINQEYTPQIERMKEIREQQLELMALTISLQEESPLKFLEKVDDVRQHVQILKQRPLPEVQPVEIYPRVSKILKEEWSRTEIGQIKNVLIPKMKISPKRMSCSWPGKDEKEVEF

相同性解析による交差性が予測される生物種
Full Name tripartite motif-containing 59
Calculated molecular weight 47 kDa
Observed molecular weight47 kDa
GenBank accession numberNM_173084
Gene Symbol TRIM59
Gene ID (NCBI) 286827
RRIDAB_2918177
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8IWR1
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

TRIM59 belongs to the tripartite motif (TRIM) family. It functions as E3 ubiquitin ligases or an adaptor protein, and plays important roles in various types of human cancers.