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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TRIM52 Polyclonal antibody (20948-1-AP)

    WB analysis using 20948-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20948-1-AP (TRIM52 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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TRIM52 Polyclonal antibody

TRIM52 Polyclonal Antibody for WB, ELISA
Cat No. 20948-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

RNF102, tripartite motif containing 52

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inNCI-H1299 cells, OVCAR-3 cells, SKOV-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20948-1-AP targets TRIM52 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TRIM52 fusion protein Ag15064 相同性解析による交差性が予測される生物種
Full Name tripartite motif-containing 52
Calculated molecular weight 297 aa, 35 kDa
Observed molecular weight35 kDa
GenBank accession numberBC007372
Gene Symbol TRIM52
Gene ID (NCBI) 84851
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96A61
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TRIM52 (Tripartite motif 52) is a member of the family of highly conserved RBCC (a RING-finger, two B-boxes, and a predicted alpha-helical Coiled-Coil domain were linked to the N-terminal region in sequence) proteins with more than 70 isoforms, which plays an important role in tumorigenesis through different signaling pathways (PMID: 35837156). TRIM52 promotes tumorigenesis in ovarian cancer by activating the nuclear factor (NF)-κB signaling pathway. Knocking-down of TRIM52 in ovarian cancer cells suppresses cell invasion, cell migration and cell proliferation, while inducing apoptosis (PMID: 30918473, 28073078).

Protocols

Product Specific Protocols
WB protocol for TRIM52 antibody 20948-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols