Validation Data Gallery
Tested Applications
| Positive WB detected in | HEK-293 cells, HeLa cells, U2OS cells, mouse colon tissue, rat colon tissue | 
| Positive IP detected in | U2OS cells | 
| Positive IHC detected in | human colon cancer tissue, human kidney tissue,  mouse colon tissue,  rat kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 | 
Recommended dilution
| Application | Dilution | 
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 | 
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate | 
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 | 
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 7 publications below | 
| WB | See 12 publications below | 
| IHC | See 9 publications below | 
| IF | See 5 publications below | 
| IP | See 4 publications below | 
| CoIP | See 1 publications below | 
Product Information
26885-1-AP targets TRIM47 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat | 
| Cited Reactivity | human, mouse, rat | 
| Host / Isotype | Rabbit / IgG | 
| Class | Polyclonal | 
| Type | Antibody | 
| Immunogen | CatNo: Ag22868 Product name: Recombinant human TRIM47 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-400 aa of BC017299 Sequence: MDELGAGIAQSRRTVALIKSAAVAERERVSRLFADAAAALQGFQTQVLGFIEEGEAAMLGRSQGDLRRQEEQRSRLSRARQNLSQVPEADSVSFLQELLALRLALEDGCGPGPGPPRELSFTKSSQAVRAVRDMLAVACVNQWEQLRGPGGNEDGPQKLDSEADAEPQDLESTNLLESEAPRDYFLKFAYIVDLDSDTADKFLQLFGTKGVKRVLCPINYPLSPTRFTHCEQVLGEGALDRGTYYWEVEIIEGWVSMGVMAEDFSPQEPYDRGRLGRNAHSCCLQWNGRSFSVWFHGLEAPLPHPFSPTVGVCLEYADRALAFYAVRDGKMSLLRRLKASRPRRGGIPASPIDPFQSRLDSHFAGLFTHRLKPAFFLESVDAHLQIGPLKKSCISVLKRR相同性解析による交差性が予測される生物種 | 
| Full Name | tripartite motif-containing 47 | 
| Calculated molecular weight | 638 aa, 70 kDa | 
| Observed molecular weight | 70 kDa | 
| GenBank accession number | BC017299 | 
| Gene Symbol | TRIM47 | 
| Gene ID (NCBI) | 91107 | 
| RRID | AB_2880669 | 
| Conjugate | Unconjugated | 
| Form | |
| Form | Liquid | 
| Purification Method | Antigen affinity purification | 
| UNIPROT ID | Q96LD4 | 
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 | 
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. | 
Background Information
TRIM47/GOA is a member of the tripartite motif RBCC/Trim domain family that contains a RING-finger domain, B-boxes, and an alpha-helical coiled-coil region. TRIM47/GOA was identified as a protein overexpressed in astrocytoma tumor cells and is thought to play a role in dedifferentiation associated with tumorigenesis.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for TRIM47 antibody 26885-1-AP | Download protocol | 
| IP protocol for TRIM47 antibody 26885-1-AP | Download protocol | 
| WB protocol for TRIM47 antibody 26885-1-AP | Download protocol | 
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols | 
Publications
| Species | Application | Title | 
|---|---|---|
| Signal Transduct Target Ther TRIM47 is a novel endothelial activation factor that aggravates lipopolysaccharide-induced acute lung injury in mice via K63-linked ubiquitination of TRAF2.
 | ||
| Mol Cell Probes TRIM47 inhibits cisplatin chemosensitivity and endoplasmic reticulum stress-induced apoptosis of ovarian cancer cells
 | ||
| iScience TRIM47 promotes head and neck squamous cell carcinoma malignant progression by degrading XAF1 through ubiquitination | ||
| Transl Oncol TRIM47 is a prognostic biomarker for gallbladder cancer and promotes tumor progression through regulating K63-linked ubiquitination of PARP1
 | ||
| Exp Brain Res Knocking down Trim47 ameliorated sevoflurane-induced neuronal cell injury and cognitive impairment in rats
 | 










