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  • KD/KO Validated

TRIM47 Polyclonal antibody

TRIM47 Polyclonal Antibody for WB, IHC, IP, ELISA
Cat No. 26885-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, CoIP, ELISA

E3 ubiquitin-protein ligase TRIM47, EC:2.3.2.27, GOA, RNF100, tripartite motif containing 47

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -


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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター(国内)に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。


保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換/補填対応を承ります。詳細はこちらをご覧ください。


Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, U2OS cells, mouse colon tissue, rat colon tissue
Positive IP detected inU2OS cells
Positive IHC detected inhuman colon cancer tissue, human kidney tissue, mouse colon tissue, rat kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

26885-1-AP targets TRIM47 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag22868

Product name: Recombinant human TRIM47 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-400 aa of BC017299

Sequence: MDELGAGIAQSRRTVALIKSAAVAERERVSRLFADAAAALQGFQTQVLGFIEEGEAAMLGRSQGDLRRQEEQRSRLSRARQNLSQVPEADSVSFLQELLALRLALEDGCGPGPGPPRELSFTKSSQAVRAVRDMLAVACVNQWEQLRGPGGNEDGPQKLDSEADAEPQDLESTNLLESEAPRDYFLKFAYIVDLDSDTADKFLQLFGTKGVKRVLCPINYPLSPTRFTHCEQVLGEGALDRGTYYWEVEIIEGWVSMGVMAEDFSPQEPYDRGRLGRNAHSCCLQWNGRSFSVWFHGLEAPLPHPFSPTVGVCLEYADRALAFYAVRDGKMSLLRRLKASRPRRGGIPASPIDPFQSRLDSHFAGLFTHRLKPAFFLESVDAHLQIGPLKKSCISVLKRR

相同性解析による交差性が予測される生物種
Full Name tripartite motif-containing 47
Calculated molecular weight 638 aa, 70 kDa
Observed molecular weight 70 kDa
GenBank accession numberBC017299
Gene Symbol TRIM47
Gene ID (NCBI) 91107
RRIDAB_2880669
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96LD4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TRIM47/GOA is a member of the tripartite motif RBCC/Trim domain family that contains a RING-finger domain, B-boxes, and an alpha-helical coiled-coil region. TRIM47/GOA was identified as a protein overexpressed in astrocytoma tumor cells and is thought to play a role in dedifferentiation associated with tumorigenesis.

Protocols

Product Specific Protocols
IHC protocol for TRIM47 antibody 26885-1-APDownload protocol
IP protocol for TRIM47 antibody 26885-1-APDownload protocol
WB protocol for TRIM47 antibody 26885-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Neoplasma

Trim47 overexpression correlates with poor prognosis in gastric cancer.

Authors - YuJian Xia
mouse,humanWB,IP,IHC

Signal Transduct Target Ther

TRIM47 is a novel endothelial activation factor that aggravates lipopolysaccharide-induced acute lung injury in mice via K63-linked ubiquitination of TRAF2.

Authors - Yisong Qian
  • KD Validated
humanWB,IHC

Mol Cell Probes

TRIM47 inhibits cisplatin chemosensitivity and endoplasmic reticulum stress-induced apoptosis of ovarian cancer cells

Authors - Jiao Zhao
  • KD Validated
humanWB,IP

iScience

TRIM47 promotes head and neck squamous cell carcinoma malignant progression by degrading XAF1 through ubiquitination

Authors - Changyun Yu
humanWB,IHC,IF,CoIP

Transl Oncol

TRIM47 is a prognostic biomarker for gallbladder cancer and promotes tumor progression through regulating K63-linked ubiquitination of PARP1

Authors - Ming Xu
  • KD Validated
ratWB

Exp Brain Res

Knocking down Trim47 ameliorated sevoflurane-induced neuronal cell injury and cognitive impairment in rats

Authors - Yingjun Zhu
  • KD Validated
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