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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using TRIM45 Monoclonal antibody (66896-1-Ig)

    WB analysis of HeLa using 66896-1-Ig (same clone as 66896-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 66896-1-Ig (TRIM45 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66896-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using TRIM45 Monoclonal antibody (66896-1-Ig)

    WB analysis of HepG2 using 66896-1-Ig (same clone as 66896-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 66896-1-Ig (TRIM45 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66896-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using TRIM45 Monoclonal antibody (66896-1-Ig)

    WB analysis of Jurkat using 66896-1-Ig (same clone as 66896-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 66896-1-Ig (TRIM45 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66896-1-PBS in a different storage buffer formulation.

TRIM45 Monoclonal antibody, PBS Only

TRIM45 Monoclonal Antibody for WB, Indirect ELISA
Cat No. 66896-1-PBS
Clone No.3A7H11

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, Indirect ELISA

Synonyms

FLJ13181, RING finger protein 99, RNF99, TRIM45, tripartite motif containing 45

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66896-1-PBS targets TRIM45 in WB, Indirect ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag28300

Product name: Recombinant human TRIM45 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 210-562 aa of BC034943

Sequence: VCQDCVVGEHREHPCDFTSNVIHKHGDSVWELLKGTQPHVEALEEALAQIHIINSALQKRVEAVAADVRTFSEGYIKAIEEHRDKLLKQLEDIRAQKENSLQLQKAQLEQLLADMRTGVEFTEHLLTSGSDLEILITKRVVVERLRKLNKVQYSTRPGVNDKIRFCPQEKAGQCRGYEIYDLHRAREKQTASFTLLCKDAAGEIMGRGGDNVQVAVVPKDKKDSPVRTMVQDNKDGTYYISYTPKEPGVYTVWVCIKEQHVQGSPFTVTVRRKHRPHSGVFHCCTFCSSGGQKTARCACGGTMPGGYLGCGHGHKGHPGHPHWSCCGKFNEKSECTWTGGQSAPRSLLRTVAL

相同性解析による交差性が予測される生物種
Full Name tripartite motif-containing 45
Calculated molecular weight562aa,62 kDa; 580aa,64 kDa
Observed molecular weight 62-64 kDa
GenBank accession numberBC034943
Gene Symbol TRIM45
Gene ID (NCBI) 80263
RRIDAB_2882225
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDQ9H8W5
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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