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Validation Data Gallery

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  • Western Blot (WB) analysis of human liver tissue using TRIM38 Polyclonal antibody (13405-1-AP)

    WB analysis of human liver using 13405-1-AP (same clone as 13405-1-PBS)

    human liver tissue were subjected to SDS PAGE followed by western blot with 13405-1-AP (TRIM38 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13405-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human colon tissue using TRIM38 Polyclonal antibody (13405-1-AP)

    WB analysis of human colon using 13405-1-AP (same clone as 13405-1-PBS)

    human colon tissue were subjected to SDS PAGE followed by western blot with 13405-1-AP (TRIM38 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13405-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using TRIM38 Polyclonal antibody (13405-1-AP)

    WB analysis of HEK-293 using 13405-1-AP (same clone as 13405-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 13405-1-AP (TRIM38 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13405-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using TRIM38 Polyclonal antibody (13405-1-AP)

    IHC staining of human liver using 13405-1-AP (same clone as 13405-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 13405-1-AP (TRIM38 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13405-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using TRIM38 Polyclonal antibody (13405-1-AP)

    IHC staining of human lung cancer using 13405-1-AP (same clone as 13405-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 13405-1-AP (TRIM38 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13405-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using TRIM38 Polyclonal antibody (13405-1-AP)

    IHC staining of human lung cancer using 13405-1-AP (same clone as 13405-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 13405-1-AP (TRIM38 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13405-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using TRIM38 Polyclonal antibody (13405-1-AP)

    IHC staining of human liver using 13405-1-AP (same clone as 13405-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 13405-1-AP (TRIM38 antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13405-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of L02 cells using TRIM38 Polyclonal antibody (13405-1-AP)

    IF Staining of L02 using 13405-1-AP (same clone as 13405-1-PBS)

    Immunofluorescent analysis of (-20℃ Ethanol) fixed L02 cells using 13405-1-AP (TRIM38 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 13405-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

TRIM38 Polyclonal antibody, PBS Only

TRIM38 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 13405-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

13405-1-PBS targets TRIM38 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag3840

Product name: Recombinant human TRIM38 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 117-466 aa of BC026930

Sequence: RAPQHKGHTTALVEDVCQGYKEKLQKAVTKLKQLEDRCTEQKLSTAMRITKWKEKVQIQRQKIRSDFKNLQCFLHEEEKSYLWRLEKEEQQTLSRLRDYEAGLGLKSNELKSHILELEEKCQGSAQKLLQNVNDTLSRSWAVKLETSEAVSLELHTMCNVSKLYFDVKKMLRSHQVSVTLDPDTAHHELILSEDRRQVTRGYTQENQDTSSRRFTAFPCVLGCEGFTSGRRYFEVDVGEGTGWDLGVCMENVQRGTGMKQEPQSGFWTLRLCKKKGYVALTSPPTSLHLHEQPLLVGIFLDYEAGVVSFYNGNTGCHIFTFPKASFSDTLRPYFQVYQYSPLFLPPPGD

相同性解析による交差性が予測される生物種
Full Name tripartite motif-containing 38
Calculated molecular weight 465 aa, 53 kDa
Observed molecular weight 53 kDa
GenBank accession numberBC026930
Gene Symbol TRIM38
Gene ID (NCBI) 10475
RRIDAB_2208307
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDO00635
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

The proinflammatory cytokines TNFα and IL-1β play central roles in many diseases, including infectious diseases, autoimmunity, and cancers. TRIM38 belongs to the TRIM protein family that is involved in various biological processes, including cell proliferation and apoptosis, innate immunity, and inflammatory response. It has been shown that TRIM38 acts as a suppressor in TOLL-like receptor (TLR)-mediated IFN-β induction by promoting degradation of TRAF6 and NAP1 through the ubiquitinproteasome system. (PMID:24434549)

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