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Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of mouse testis tissue using TRIM36 Polyclonal antibody (25913-1-AP)

    IHC staining of mouse testis using 25913-1-AP (same clone as 25913-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 25913-1-AP (TRIM36 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25913-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using TRIM36 Polyclonal antibody (25913-1-AP)

    IHC staining of mouse testis using 25913-1-AP (same clone as 25913-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 25913-1-AP (TRIM36 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25913-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat testis tissue using TRIM36 Polyclonal antibody (25913-1-AP)

    IHC staining of rat testis using 25913-1-AP (same clone as 25913-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 25913-1-AP (TRIM36 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25913-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat testis tissue using TRIM36 Polyclonal antibody (25913-1-AP)

    IHC staining of rat testis using 25913-1-AP (same clone as 25913-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 25913-1-AP (TRIM36 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25913-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse testis tissue using TRIM36 Polyclonal antibody (25913-1-AP)

    IF Staining of mouse testis using 25913-1-AP (same clone as 25913-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse testis tissue using TRIM36 antibody (25913-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25913-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse testis tissue using TRIM36 Polyclonal antibody (25913-1-AP)

    IF Staining of mouse testis using 25913-1-AP (same clone as 25913-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse testis tissue using TRIM36 antibody (25913-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25913-1-PBS in a different storage buffer formulation.

TRIM36 Polyclonal antibody, PBS Only

TRIM36 Polyclonal Antibody for IHC, IF-P, Indirect ELISA
Cat No. 25913-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, IF-P, Indirect ELISA

Synonyms

E3 ubiquitin-protein ligase TRIM36, EC:2.3.2.27, RING-type E3 ubiquitin transferase TRIM36, RNF98, tripartite motif containing 36

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

25913-1-PBS targets TRIM36 in IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag23240

Product name: Recombinant human TRIM36 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 166-215 aa of BC130334

Sequence: ESTKSCMDCSASYCNECFKIHHPWGTIKAQHEYVGPTTNFRPKILMCPEH

相同性解析による交差性が予測される生物種
Full Name tripartite motif-containing 36
Observed molecular weight83 kDa
GenBank accession numberBC130334
Gene Symbol TRIM36
Gene ID (NCBI) 55521
RRIDAB_2880293
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NQ86
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

TRIM36, a member of the tripartite motif (TRIM) family of RING-containing proteins, also known as Haprin, is a microtubule-associated E3 ubiquitin ligase that plays a role in cytoskeletal organization, which was first discovered for its abundance in testis and found to be implicated in the spermatozoa acrosome reaction (PMID: 35053362, 30944633). TRIM36 was reported as a novel androgen signaling target gene and is upregulated in prostate cancer (PMID: 29449534).

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