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Validation Data Gallery

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  • Western Blot (WB) analysis of A431 cells using TRIM35 Polyclonal antibody (19806-1-AP)

    WB analysis of A431 using 19806-1-AP

    WB result of TRIM35 antibody (19806-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TRIM35 transfected A431 cells.

  • Western Blot (WB) analysis of various lysates using TRIM35 Polyclonal antibody (19806-1-AP)

    WB analysis using 19806-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 19806-1-AP (TRIM35 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver tissue using TRIM35 Polyclonal antibody (19806-1-AP)

    IHC staining of human liver using 19806-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 19806-1-AP (TRIM35 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver tissue using TRIM35 Polyclonal antibody (19806-1-AP)

    IHC staining of human liver using 19806-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 19806-1-AP (TRIM35 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse liver tissue using TRIM35 Polyclonal antibody (19806-1-AP)

    IHC staining of mouse liver using 19806-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 19806-1-AP (TRIM35 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using TRIM35 Polyclonal antibody (19806-1-AP)

    IF Staining of U2OS using 19806-1-AP

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using TRIM35 antibody (19806-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

  • Featured Product
  • KD/KO Validated

TRIM35 Polyclonal antibody

TRIM35 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 19806-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

E3 ubiquitin-protein ligase TRIM35, EC:2.3.2.27, HLS5, KIAA1098, tripartite motif containing 35

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inH9C2 cells, A431 cells, HEK-293 cells
Positive IHC detected inhuman liver tissue, mouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU2OS cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

19806-1-AP targets TRIM35 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag13584

Product name: Recombinant human TRIM35 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-206 aa of BC018337

Sequence: MERSPDVSPGPSRSFKEELLCAVCYDPFRDAVTLRCGHNFCRGCVSRCWEVQVSPTCPVCKDRASPADLRTNHTLNNLVEKLLREEAEGARWTSYRFSRVCRLHRGQLSLFCLEDKELLCCSCQADPRHQGHRVQPVKDTAHDFRVRSLIAEERRNFLPTHQWIVTKTRLQTSSPNLQSRRQGQVQEHGACTAGEGQGLLGHAALL

相同性解析による交差性が予測される生物種
Full Name tripartite motif-containing 35
Calculated molecular weight 57 kDa
Observed molecular weight45~50 kDa
GenBank accession numberBC018337
Gene Symbol TRIM35
Gene ID (NCBI) 23087
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UPQ4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Tripartite motif 35 (TRIM35), also called hemopoietic lineage switch (Hls5), is a member of the RING finger, B box, coiled-coil (RBCC), or TRIM family E3 ligases expressed in a wide variety of hemopoietic cell types, including fetal liver progenitors. TRIM35 is reported to be a tumor suppressor gene and is expressed at low levels in several types of cancer (PMID: 34124276, 35081724). Western detected a band at 45-50 kDa possibly due to the high proline content.

Protocols

Product Specific Protocols
IF protocol for TRIM35 antibody 19806-1-APDownload protocol
IHC protocol for TRIM35 antibody 19806-1-APDownload protocol
WB protocol for TRIM35 antibody 19806-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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