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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

  • Featured Product
  • KD/KO Validated

TRIM33 Polyclonal antibody

TRIM33 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 55374-1-AP

Synonyms

Ectodermin homolog, KIAA1113, Protein Rfg7, PTC7, RET fused gene 7 protein, RFG7, TF1G, TIF1 gamma, TIF1G, TIF1GAMMA, TIFGAMMA, TRIM33, tripartite motif containing 33

Validation Data Gallery

Applications:
  • WB analysis of COLO 320 using 55374-1-AP

    WB analysis of COLO 320 using 55374-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 55374-1-AP (TRIM33 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis of PC-3 using 55374-1-AP

    WB analysis of PC-3 using 55374-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 55374-1-AP (TRIM33 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis of MCF-7 using 55374-1-AP

    WB analysis of MCF-7 using 55374-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 55374-1-AP (TRIM33 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung cancer using 55374-1-AP

    IHC staining of human lung cancer using 55374-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 55374-1-AP (TRIM33 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human lung cancer using 55374-1-AP

    IHC staining of human lung cancer using 55374-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 55374-1-AP (TRIM33 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inCOLO 320 cells, MCF-7 cells, PC-3 cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

55374-1-AP targets TRIM33 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name tripartite motif-containing 33
Calculated molecular weight 122 kDa
Observed molecular weight 140-150 kDa
GenBank accession numberNM_015906
Gene symbol TRIM33
Gene ID (NCBI) 51592
RRIDAB_11183035
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TRIM33, also named as KIAA1113, RFG7 and TIF1G, belongs to the TRIM/RBCC family. It acts as an E3 ubiquitin-protein ligase. TRIM33 promotes SMAD4 ubiquitination, nuclear exclusion and degradation via the ubiquitin proteasome pathway. It may act as a transcriptional repressor. It inhibits the transcriptional response to TGF-beta/BMP signaling cascade. TRIM33 plays a role in the control of cell proliferation. Its association with SMAD2 and SMAD3, TRIM33 stimulates erythroid differentiation of hematopoietic stem/progenitor. Defects in TRIM33 are a cause of thyroid papillary carcinoma (TPC). This antibody is specific to TRIM33. The calculated molecular weight of TRIM33 is 122kDa, but the molecular weight of detected is about 140-150 kDa as the same as the result from the paper(PMID:25639486;26592194).

Protocols

Product Specific Protocols
WB protocol for TRIM33 antibody 55374-1-APDownload protocol
IHC protocol for TRIM33 antibody 55374-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

Nat Commun

Identification of proximal SUMO-dependent interactors using SUMO-ID.

Authors - Orhi Barroso-Gomila
View Article
humanWB,IHC

Technol Cancer Res Treat

Downregulation of TRIM33 Promotes Survival and Epithelial-Mesenchymal Transition in Gastric Cancer

Authors - Fang Wang
  • KD Validated
View Article
humanWB,IF,IP

Exp Dermatol

Nuclear translocation of PKM2 mediates keratinocyte metabolic reprogramming in psoriasis

Authors - Luting Yang
View Article