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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TRIM29 Polyclonal antibody (17542-1-AP)

    WB analysis using 17542-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17542-1-AP (TRIM29 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of BGC-823 cells using TRIM29 Polyclonal antibody (17542-1-AP)

    WB analysis of BGC-823 using 17542-1-AP

    BGC-823 cells were subjected to SDS PAGE followed by western blot with 17542-1-AP (TRIM29 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A431 cells using TRIM29 Polyclonal antibody (17542-1-AP)

    WB analysis of A431 using 17542-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 17542-1-AP (TRIM29 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A431 cells using TRIM29 Polyclonal antibody (17542-1-AP)

    WB analysis of A431 using 17542-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 17542-1-AP (TRIM29 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A431 cells using TRIM29 Polyclonal antibody (17542-1-AP)

    WB analysis of A431 using 17542-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 17542-1-AP (TRIM29 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using TRIM29 Polyclonal antibody (17542-1-AP)

    IHC staining of human skin cancer using 17542-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 17542-1-AP (TRIM29 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using TRIM29 Polyclonal antibody (17542-1-AP)

    IHC staining of human skin cancer using 17542-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 17542-1-AP (TRIM29 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human skin tissue using TRIM29 Polyclonal antibody (17542-1-AP)

    IHC staining of human skin using 17542-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 17542-1-AP (TRIM29 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human skin tissue using TRIM29 Polyclonal antibody (17542-1-AP)

    IHC staining of human skin using 17542-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 17542-1-AP (TRIM29 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human skin tissue using TRIM29 Polyclonal antibody (17542-1-AP)

    IHC staining of human skin using 17542-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin using 17542-1-AP (TRIM29 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human skin tissue using TRIM29 Polyclonal antibody (17542-1-AP)

    IHC staining of human skin using 17542-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin using 17542-1-AP (TRIM29 antibody) at dilution of 1:100 (under 40x lens).

  • Featured Product
  • KD/KO Validated

TRIM29 Polyclonal antibody

TRIM29 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 17542-1-AP
Publications(20)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF, IP, ELISA

Synonyms

Ataxia telangiectasia group D-associated protein, ATDC, tripartite motif containing 29, Tripartite motif-containing protein 29

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inBxPC-3 cells, A431 cells, BGC-823 cells
Positive IHC detected inhuman skin cancer tissue, human skin tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:20000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17542-1-AP targets TRIM29 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag11600

Product name: Recombinant human TRIM29 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 239-588 aa of BC017352

Sequence: DQTCICYLCMFQEHKNHSTVTVEEAKAEKETELSLQKEQLQLKIIEIEDEAEKWQKEKDRIKSFTTNEKAILEQNFRDLVRDLEKQKEEVRAALEQREQDAVDQVKVIMDALDERAKVLHEDKQTREQLHSISDSVLFLQEFGALMSNYSLPPPLPTYHVLLEGEGLGQSLGNFKDDLLNVCMRHVEKMCKADLSRNFIERNHMENGGDHRYVNNYTNSFGGEWSAPDTMKRYSMYLTPKGGVRTSYQPSSPGRFTKETTQKNFNNLYGTKGNYTSRVWEYSSSIQNSDNDLPVVQGSSSFSLKGYPSLMRSQSPKAQPQTWKSGKQTMLSHYRPFYVNKGNGIGSNEAP

相同性解析による交差性が予測される生物種
Full Name tripartite motif-containing 29
Calculated molecular weight 588 aa, 66 kDa
Observed molecular weight65 kDa
GenBank accession numberBC017352
Gene Symbol TRIM29
Gene ID (NCBI) 23650
RRIDAB_2272412
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ14134
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TRIM29(Tripartite motif-containing protein 29) is also named as ATDC( Ataxia telangiectasia group D-associated protein). ATDC protein physically interacted with the intermediate filament protein vimentin, a protein kinase C substrate and colocalizing protein, and with an inhibitor of protein kinase C, PKCI1 (PMID:7644499). Knockdown of TRIM29 in airway epithelial cells enhances type I interferon production, and in human nasopharyngeal carcinoma cells results in almost complete Epstein-Barr virus clearance. TRIM29 is also highly induced by cytosolic double-stranded DNA in myeloid dendritic cells (PMID: 29038422). Western detected both full-length TRIM29 (65 kDa) and a truncated variant of TRIM29 with a molecular weight of about 50 kDa (PMID: 26095369).

Protocols

Product Specific Protocols
IHC protocol for TRIM29 antibody 17542-1-APDownload protocol
WB protocol for TRIM29 antibody 17542-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouse,humanWB,IP,IHC

Mol Cancer

CircIL4R activates the PI3K/AKT signaling pathway via the miR-761/TRIM29/PHLPP1 axis and promotes proliferation and metastasis in colorectal cancer.

Authors - Tao Jiang
  • KD Validated
View Article
humanWB

Cancer Commun (Lond)

HMMR alleviates endoplasmic reticulum stress by promoting autophagolysosomal activity during endoplasmic reticulum stress-driven hepatocellular carcinoma progression

Authors - Lin He
  • KD Validated
View Article
humanWB

Drug Resist Updat

TRIM29 facilitates gemcitabine resistance via MEK/ERK pathway and is modulated by circRPS29/miR-770-5p axis in PDAC

Authors - Wenjie Huang
View Article
WB

Exp Mol Med

The long non-coding RNA-encoded microprotein MKKS3 drives colorectal cancer chemoresistance

Authors - Hui-Fang Hu
View Article

Sci Adv

TRIM29-dependent AKR1B10 degradation ameliorates MASH.

Authors - Zhengcai Ma
View Article
humanWB

Research (Wash D C)

tRF-AspGTC Promotes Intracranial Aneurysm Formation by Controlling TRIM29-Mediated Galectin-3 Ubiquitination

Authors - Chao Wang
View Article
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