Validation Data Gallery

Filter: AllWBIPIHCIF/ICCFC (Intra)

WB analysis of HeLa using 15202-1-AP
WB result of KAP1 antibody (15202-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-KAP1 transfected HeLa cells.
WB analysis of HeLa using 15202-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 15202-1-AP (KAP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis using 15202-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 15202-1-AP (KAP1 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 15202-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 15202-1-AP (KAP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of rat spleen using 15202-1-AP
rat spleen tissue were subjected to SDS PAGE followed by western blot with 15202-1-AP (KAP1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of mouse testis using 15202-1-AP
mouse testis tissue were subjected to SDS PAGE followed by western blot with 15202-1-AP (KAP1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 15202-1-AP
IP result of anti-KAP1 (IP:15202-1-AP, 4ug; Detection:15202-1-AP 1:1000) with HeLa cells lysate 1200ug.
IHC staining of human lung cancer using 15202-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15202-1-AP (KAP1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 15202-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15202-1-AP (KAP1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon using 15202-1-AP
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 15202-1-AP (KAP1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HeLa using 15202-1-AP
Immunofluorescent analysis of Hela cells, using TRIM28 antibody 15202-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
IF Staining of HeLa using 15202-1-AP
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using KAP1 antibody (15202-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).
IF Staining of HeLa using 15202-1-AP
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using KAP1 antibody (15202-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
FC experiment of HeLa using 15202-1-AP
1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human KAP1 (15202-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug rabbit IgG isotype control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

Featured Product
KD/KO Validated

KAP1 Polyclonal antibody

KAP1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Cat No. 15202-1-AP

Publications(61)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ChIP, ELISA

Synonyms

TRIM28, E3 SUMO-protein ligase TRIM28, EC:2.3.2.27, KAP 1, KAP-1

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488 CoraLite® Plus 647 CoraLite®555

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (61)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HeLa cells, mouse testis tissue, HepG2 cells, L02 cells
Positive IP detected in	HeLa cells
Positive IHC detected in	human lung cancer tissue, human colon tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells
Positive FC (Intra) detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:20000-1:100000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:1000-1:4000
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 12 publications below
WB	See 49 publications below
IHC	See 9 publications below
IF	See 6 publications below
IP	See 8 publications below
CoIP	See 6 publications below
ChIP	See 4 publications below

Product Information

15202-1-AP targets KAP1 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, pig, monkey
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag7350 Product name: Recombinant human KAP1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 484-835 aa of BC004978 Sequence: KVPRVSLERLDLDLTADSQPPVFKVFPGSTTEDYNLIVIERGAAAAATGQPGTAPAGTPGAPPLAGMAIVKEEETEAAIGAPPTATEGPETKPVLMALAEGPGAEGPRLASPSGSTSSGLEVVAPEGTSAPGGGPGTLDDSATICRVCQKPGDLVMCNQCEFCFHLDCHLPALQDVPGEEWSCSLCHVLPDLKEEDGSLSLDGADSTGVVAKLSPANQRKCERVLLALFCHEPCRPLHQLATDSTFSLDQPGGTLDLTLIRARLQEKLSPPYSSPQEFAQDVGRMFKQFNKLTEDKADVQSIIGLQRFFETRMNEAFGDTKFSAVLVEPPPMSLPGAGLSSQELSGGPGDGP 相同性解析による交差性が予測される生物種
Full Name	tripartite motif-containing 28
Calculated molecular weight	89 kDa
Observed molecular weight	100 kDa
GenBank accession number	BC004978
Gene Symbol	KAP1
Gene ID (NCBI)	10155
RRID	AB_2209890
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q13263
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

KAP1, also named as TRIM28 or RNF96, is a 835 amino acid protein, which contain one RING-type zinc finger, one PHD-type zinc finger, one bromo domain and two B box-type zinc fingers. KAP1 localizes in the nucleus and Belongs to the TRIM/RBCC family. KAP1 is a nuclear corepressor for KRAB domain-containing zinc finger proteins and mediates gene silencing by recruiting CHD3, a subunit of the nucleosome remodeling and deacetylation (NuRD) complex, and SETDB1 to the promoter regions of KRAB target genes. KAP1 is expressed in all tissues tested including spleen, thymus, prostate, testis, ovary, small intestine, colon and peripheral blood leukocytes. The calcualted molecular weight of KAP1 is 89 kDa, but modified KAP1 is about 100 kDa. (PMID: 18590578 )

Protocols

Product Specific Protocols
IF protocol for KAP1 antibody 15202-1-AP	Download protocol
IHC protocol for KAP1 antibody 15202-1-AP	Download protocol
IP protocol for KAP1 antibody 15202-1-AP	Download protocol
WB protocol for KAP1 antibody 15202-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	Mol Cancer ZDHHC20 mediated S-palmitoylation of fatty acid synthase (FASN) promotes hepatocarcinogenesis Authors - Yaqi Mo View Article
human	WB	Cancer Discov Transcription Elongation Machinery Is a Druggable Dependency and Potentiates Immunotherapy in Glioblastoma Stem Cells Authors - Zhixin Qiu View Article
human	WB,CoIP	Gut Targeting NEK9 synergises with immunotherapy in hepatocellular carcinoma by remodelling the immunosuppressive microenvironment Authors - Guofang Lu View Article
human	WB,IHC	Sci Bull (Beijing) TRIM28 protects CARM1 from proteasome-mediated degradation to prevent colorectal cancer metastasis Authors - Jinyuan Cui View Article
human	WB,IP,CoIP	Nat Commun ZNF274 constrains lineage plasticity and drives intrinsic resistance to CDK7 inhibitors in pancreatic cancer. Authors - Jessica E. Gianopulos View Article
human	WB	Nat Commun URI alleviates tyrosine kinase inhibitors-induced ferroptosis by reprogramming lipid metabolism in p53 wild-type liver cancers Authors - Zhiwen Ding View Article

Validation Data Gallery

WB analysis of HeLa using 15202-1-AP

WB analysis of HeLa using 15202-1-AP

WB analysis using 15202-1-AP

WB analysis of HeLa using 15202-1-AP

WB analysis of rat spleen using 15202-1-AP

WB analysis of mouse testis using 15202-1-AP

IP experiment of HeLa using 15202-1-AP

IHC staining of human lung cancer using 15202-1-AP

IHC staining of human lung cancer using 15202-1-AP

IHC staining of human colon using 15202-1-AP

IF Staining of HeLa using 15202-1-AP

IF Staining of HeLa using 15202-1-AP

IF Staining of HeLa using 15202-1-AP

FC experiment of HeLa using 15202-1-AP

KAP1 Polyclonal antibody

KAP1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

ZDHHC20 mediated S-palmitoylation of fatty acid synthase (FASN) promotes hepatocarcinogenesis

Transcription Elongation Machinery Is a Druggable Dependency and Potentiates Immunotherapy in Glioblastoma Stem Cells

Targeting NEK9 synergises with immunotherapy in hepatocellular carcinoma by remodelling the immunosuppressive microenvironment

TRIM28 protects CARM1 from proteasome-mediated degradation to prevent colorectal cancer metastasis

ZNF274 constrains lineage plasticity and drives intrinsic resistance to CDK7 inhibitors in pancreatic cancer.

URI alleviates tyrosine kinase inhibitors-induced ferroptosis by reprogramming lipid metabolism in p53 wild-type liver cancers

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