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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TRIM24 Monoclonal antibody (66324-1-Ig)

    WB analysis using 66324-1-Ig (same clone as 66324-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66324-1-Ig (TRIM24 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66324-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using TRIM24 Monoclonal antibody (66324-1-Ig)

    WB analysis of HeLa using 66324-1-Ig (same clone as 66324-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 66324-1-Ig (TRIM24 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66324-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using TRIM24 Monoclonal antibody (66324-1-Ig)

    WB analysis of HEK-293 using 66324-1-Ig (same clone as 66324-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 66324-1-Ig (TRIM24 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66324-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using TRIM24 Monoclonal antibody (66324-1-Ig)

    IHC staining of human breast cancer using 66324-1-Ig (same clone as 66324-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66324-1-Ig (TRIM24 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66324-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using TRIM24 Monoclonal antibody (66324-1-Ig)

    IHC staining of human lung cancer using 66324-1-Ig (same clone as 66324-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66324-1-Ig (TRIM24 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66324-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using TRIM24 Monoclonal antibody (66324-1-Ig)

    IHC staining of human lung cancer using 66324-1-Ig (same clone as 66324-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66324-1-Ig (TRIM24 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66324-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using TRIM24 Monoclonal antibody (66324-1-Ig)

    IHC staining of human liver cancer using 66324-1-Ig (same clone as 66324-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66324-1-Ig (TRIM24 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66324-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using TRIM24 Monoclonal antibody (66324-1-Ig)

    IHC staining of human liver cancer using 66324-1-Ig (same clone as 66324-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66324-1-Ig (TRIM24 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66324-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using TRIM24 Monoclonal antibody (66324-1-Ig)

    IHC staining of human breast cancer using 66324-1-Ig (same clone as 66324-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66324-1-Ig (TRIM24 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66324-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using TRIM24 Monoclonal antibody (66324-1-Ig)

    IF Staining of HepG2 using 66324-1-Ig (same clone as 66324-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using TRIM24 antibody (66324-1-Ig, Clone: 1D6D9 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 66324-1-PBS in a different storage buffer formulation.

TRIM24 Monoclonal antibody, PBS Only

TRIM24 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 66324-1-PBS
Clone No.1D6D9

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

1D6D9, E3 ubiquitin-protein ligase TRIM24, EC:2.3.2.27, RING-type E3 ubiquitin transferase TIF1-alpha, TIF1

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66324-1-PBS targets TRIM24 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag5417

Product name: Recombinant human TRIM24 protein

Source: e coli.-derived, T-HIS

Tag: 6*His

Domain: 701-1050 aa of BC028689

Sequence: SKPAGADSTHKVPVVMLEPIRIKQENSGPPENYDFPVVIVKQESDEESRPQNANYPRSILTSLLLNSSQSSTSEETVLRSDAPDSTGDQPGLHQDNSSNGKSEWLDPSQKSPLHVGETRKEDDPNEDWCAVCQNGGELLCCEKCPKVFHLSCHVPTLTNFPSGEWICTFCRDLSKPEVEYDCDAPSHNSEKKKTEGLVKLTPIDKRKCERLLLFLYCHEMSLAFQDPVPLTVPDYYKIIKNPMDLSTIKKRLQEDYSMYSKPEDFVADFRLIFQNCAEFNEPDSEVANAGIKLENYFEELLKNLYPEKRFPKPEFRNESEDNKFSDDSDDDFVQPRKKRLKSIEERQLLK

相同性解析による交差性が予測される生物種
Full Name tripartite motif-containing 24
Calculated molecular weight 117 kDa
Observed molecular weight 116 kDa
GenBank accession numberBC028689
Gene Symbol TRIM24
Gene ID (NCBI) 8805
RRIDAB_2881705
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDO15164
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

TRIM24, also named as RNF82, TIF1 and TIF1A, interacts selectively in vitro with the AF2-activating domain of the estrogen receptors. Association with DNA-bound estrogen receptors, it requires the presence of estradiol. It is a regulation protein of P53. Defects in TRIM24 are a cause of thyroid papillary carcinoma (TPC) which is a common tumor of the thyroid that typically arises as an irregular, solid or cystic mass from otherwise normal thyroid tissue.

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