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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TRIM21 Recombinant antibody (83530-3-RR)

    WB analysis using 83530-3-RR (same clone as 83530-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83530-3-RR (TRIM21 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83530-3-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using TRIM21 Recombinant antibody (83530-3-RR)

    IHC staining of human colon cancer using 83530-3-RR (same clone as 83530-3-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 83530-3-RR (TRIM21 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83530-3-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using TRIM21 Recombinant antibody (83530-3-RR)

    IF Staining of A549 using 83530-3-RR (same clone as 83530-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using TRIM21 antibody (83530-3-RR, Clone: 240491B2 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83530-3-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using TRIM21 Recombinant antibody (83530-3-RR)

    FC experiment of HeLa using 83530-3-RR (same clone as 83530-3-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug TRIM21 Recombinant antibody (83530-3-RR, Clone:240491B2) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83530-3-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83530-3-RR

    Affinity and Kinetic Characterization of 83530-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83530-3-RR against Human TRIM21 were performed. The affinity constant is 11 nM.

TRIM21 Recombinant monoclonal antibody, PBS Only

TRIM21 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Cat No. 83530-3-PBS
Clone No.240491B2

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Synonyms

240491B2, 52 kDa ribonucleoprotein autoantigen Ro/SS-A, E3 ubiquitin-protein ligase TRIM21, EC:2.3.2.27, RING finger protein 81

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83530-3-PBS targets TRIM21 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag28377

Product name: Recombinant human TRIM21 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 176-475 aa of BC010861

Sequence: SRIHAEFVQQKNFLVEEEQRQLQELEKDEREQLRILGEKEAKLAQQSQALQELISELDRRCHSSALELLQEVIIVLERSESWNLKDLDITSPELRSVCHVPGLKKMLRTCAVHITLDPDTANPWLILSEDRRQVRLGDTQQSIPGNEERFDSYPMVLGAQHFHSGKHYWEVDVTGKEAWDLGVCRDSVRRKGHFLLSSKSGFWTIWLWNKQKYEAGTYPQTPLHLQVPPCQVGIFLDYEAGMVSFYNITDHGSLIYSFSECAFTGPLRPFFSPGFNDGGKNTAPLTLCPLNIGSQGSTDY

相同性解析による交差性が予測される生物種
Full Name tripartite motif-containing 21
Calculated molecular weight 475 aa, 54 kDa
Observed molecular weight52 kDa
GenBank accession numberBC010861
Gene Symbol TRIM21
Gene ID (NCBI) 6737
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purfication
UNIPROT IDP19474
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

TRIM21 has multiple N-terminal zinc finger motifs(has E3 ligase activity), a central leucine zipper, and a potential N-glycosylation site. TRIM21 is a E3 ubiquitin-protein ligase. It can form a ubiquitin ligase complex with E2 UBE2D2, which function not only for the uniquitination of USP4 and IKBKB but also for its self-ubiquitination. It has a role in the regulation of the cell cycle progression and could enhance the decapping activity of DCP2. It can exist in all mammalian cells as a ribonucleoprotein particle , which composed of a single polypeptide and 1 of 4 small RNA molecules. TRIM21 exists various isoforms and range molecular weight of isoforms is about 42-45kDa and 49-54 kDa.

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